| 自制纤维素金属螯合亲和膜的部分吸附性能及纯化牛血SOD |
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| 引用本文： | 刘建荣,张昊,顾雅君,赵晓瑜. 自制纤维素金属螯合亲和膜的部分吸附性能及纯化牛血SOD[J]. 河北大学学报(自然科学版), 2009, 29(4): 427. DOI: 10.3969/j.issn.1000-1565.2009.04.022 |
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| 作者姓名： | 刘建荣 张昊 顾雅君 赵晓瑜 |
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| 作者单位： | 河北省生物工程技术研究中心,河北,保定,071002;河南双汇投资发展股份有限公司,河南,漯河,462000;河北大学,生命科学学院,河北,保定,071002 |
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| 基金项目： | 河北省科技研究与发展指导计划项目，河北省教育厅科学研究计划项目 |
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| 摘 要： | ×10-6 g/cm2,但蛋白解吸率前者低于后者,表明一定浓度的NaCl有助于亲和膜对蛋白质的吸附,同时也使被吸附的蛋白的解吸率有所降低.吸附-解吸方式(静态法)分离纯化SOD,解吸样品比活达8 770 U/mg,纯度为81%;吸附-洗脱方式(动态法)分离纯化SOD,穿柱流出样品比活达12 418 U/mg,纯度为85%.自制纤维素亲和膜有较好的吸附性能和纯化效果,重复使用性能良好,制备和纯化过程简便易行,具有广阔应用前景.
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| 关 键 词： | 亲和膜 金属螯合 纤维素 超氧化物歧化酶(SOD) |
Partial Adsorption Characteristics of Self-made Cellulose Metal-chelated Affinity Membraneand Its Application for Purification of Bovine SOD from Blood |
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| LIU Jian-rong,ZHANG Hao,GU Ya-jun,ZHAO Xiao-yu. Partial Adsorption Characteristics of Self-made Cellulose Metal-chelated Affinity Membraneand Its Application for Purification of Bovine SOD from Blood[J]. Journal of Hebei University (Natural Science Edition), 2009, 29(4): 427. DOI: 10.3969/j.issn.1000-1565.2009.04.022 |
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| Authors: | LIU Jian-rong ZHANG Hao GU Ya-jun ZHAO Xiao-yu |
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| Affiliation: | 1.Hebei Province Bioengineering Technological Research Center;Baoding 071002;China;2.Henan Shuanghui Investment and Development Company Limited;Luohe 462000;3.College of Life Sciences;Hebei University;China |
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| Abstract: | To study adsorption characteristics for metal ion(Cu2+,Ni2+,Fe3+) and proteins of self-made cellulose metal-chelated affinity membrane,and establish an efficient and convenient method for purifing superoxide dismutase(SOD),affinity membrane with Cu2+,Ni2+ and Fe3+ was prepared using cellulose filter paper as a matrix.Adsorption characteristics for proteins of affinity membrane were studied by adsorption-desorption experiments of ovalbumin.The adsorption capacity of affinity membrane for Cu2+ was the largest... |
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| Keywords: | affinity membrane metal chelating cellulose superoxide dismutase(SOD) |
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