脂肪乳对血液细胞CBC参数及Diff分类参数检测的影响

目的 观察脂肪乳对血液细胞分析结果的影响.方法 在体外将健康志愿者血液样本以及生理盐水与一定比例的脂肪乳混合,配制系列浓度的血液/脂肪乳混合液以及生理盐水/脂肪乳混合液,于血液细胞分析仪上进行测试,观察不同浓度脂肪乳对CBC参数、Diff分类参数的影响.结果 随着脂肪乳浓度的增高,血液/脂肪乳混合液的CBC参数测定值渐进升高,Diff分类参数中的淋巴细胞分类比例呈下降趋势而单核细胞比例逐渐增高；而生理盐水/脂肪乳混合液中白细胞、血红蛋白和PLT-O明显升高.结论 脂肪乳会对血液细胞分析仪多个参数的测定产生影响,可以通过测定结果的一些特征筛选可疑含脂肪乳标本.

Infludence of fat emulsion on blood cell CBC parameters and Diff classification parameters analysis

Objective To observe influence of emulsion on the analysis results of blood cells. Methods Blood sample from healthy volunteer and normal saline were mixed with fat emulsion at a schedule proportion in vitro. Serial concentrations of blood fat emulsion mixture and saline intralipid mixture were determined with blood cell analyzers. The CBC parameters and Diff classification parameters were analyzed. Results CBC parameters and MON% in Diff classification of blood fat emulsion mixture gradually elevated with the increasing of emulsion concentration while LYMPH % in Diff classification parameter declined. At he sime time, WBC, HGB and PLT-O in saline-Intralipid mixture increased significantly. Conclusions Various datas of the blood cell analyzer can be disturbed by fat emulsion in blood samples and the questionable samples can be screened by scatterplot.