溃疡性结肠炎患者外周血Tim-4 mRNA表达水平的变化及意义

目的 探究溃疡性结肠炎患者外周血Tim-4 mRNA的表达变化及临床意义。方法 选取2015年2月~2019年4月我院收治的溃疡性结肠炎患者38例作为观察组，另选取同期来我院体检的健康者30名作为对照组，采用荧光定量PCR测定外周血Tim-4 mRNA表达水平，比较两组Tim-4 mRNA、C反应蛋白（CRP）、红细胞沉降率（ESR）表达水平及观察组Tim-4 mRNA与CRP和ESR的相关性。结果 观察组Tim-4 mRNA、CRP、ESR表达均高于对照组（0.79±0.37）vs（0.33±0.20）]、（6.08±3.21）mg/L vs（0.99±0.87）mg/L]、（19.12±12.13）mm/h vs（5.58±4.10）mm/h]，差异有统计学意义（P＜0.05）。观察组外周血单个核细胞Tim-4 mRNA表达水平与CRP呈正相关（r=0.376，P＜0.05），而与ESR无相关性（r=0.077，P＞0.05）。结论 溃疡性结肠炎患者Tim-4 mRNA异常上调，并与CRP密切相关，可能参与溃疡性结肠炎的致病过程。

Changes and Significance of Tim-4 mRNA Expression in Peripheral Blood of Patients with Ulcerative Colitis

Objective To investigate the expression and clinical significance of Tim-4 mRNA expression in peripheral blood of patients with ulcerative colitis.Methods A total of 38 patients with ulcerative colitis admitted to our hospital from February 2015 to April 2019 were enrolled as the observation group. 30 healthy subjects from our hospital for the same period were selected as the control group. The expression of Tim-4 mRNA in peripheral blood was determined by real-time PCR. The expressions of Tim-4 mRNA, C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) in the two groups and the correlation between Tim-4 mRNA and CRP and ESR in the observation group were compared.Results The expressions of Tim-4 mRNA, CRP and ESR in the observation group were higher than those in the control group (0.79±0.37) vs (0.33±0.20)], (6.08±3.21) mg/L vs (0.99±0.87) mg/L], (19.12±12.13) mm/h vs (5.58±4.10) mm/h], the difference was statistically significant (P<0.05). The expression of Tim-4 mRNA in peripheral blood mononuclear cells of the observation group was positively correlated with CRP (r=0.376, P<0.05), but not correlated with ESR (r=0.077, P>0.05). Conclusion The abnormal expression of Tim-4 mRNA in patients with ulcerative colitis is closely related to CRP and may be involved in the pathogenesis of ulcerative colitis.