| 人参水提物对MPP^＋诱导的SH-SY5Y细胞凋亡的保护作用 |
| |
| 引用本文： | 胡胜全,周红祖,刘塔斯,余惠旻,杨大坚,陈新滋.人参水提物对MPP^＋诱导的SH-SY5Y细胞凋亡的保护作用[J].中南药学,2008,6(3):257-260. |
| |
| 作者姓名： | 胡胜全 周红祖 刘塔斯 余惠旻 杨大坚 陈新滋 |
| |
| 作者单位： | [1]深圳市中药药学及分子药理学研究重点实验室,广东深圳518057; [2]湖南中医药大学药学院,长沙410007; [3]深圳市二医院,广东深圳518035 |
| |
| 基金项目： | 国家重点基础研究发展计划(973计划) |
| |
| 摘 要： | 目的研究人参水提物（WEG）对四氢吡啶离子（MPP^＋）诱导SH-SY5Y细胞凋亡的影响。方法体外培养SH-SY5Y细胞,建立MPP＋致SH-SY5Y细胞凋亡的模型,同时给予WEG干预。应用MTT比色法检测细胞活力;Hoechst33258染色观察细胞形态学变化,流式细胞仪检测细胞凋亡率。结果0.5mmol·L^-1MPP^＋作用于SH-SY5Y细胞60h,MTT值降低到（51.97%±2.46%）（P〈0.01）,Hoechst 33258染色可见明显的颗粒状和固缩状荧光,凋亡率增加到（40.52%±1.68%）（P〈0.01）,而WEG则能明显改善SH-SY5Y细胞的凋亡特性,显著升高其MTT值,降低凋亡率（P〈0.05）。结论人参水提物对MPP＋诱导的SH-SY5Y细胞凋亡具有显著的保护作用。
|
| 关 键 词： | SH-SY5Y细胞 四氢吡啶离子 人参水提物 细胞凋亡 |
Protective effect of water extracts of Ginseng on SH-SY5Y cell apoptosis induced by MPP+ |
| |
| HU Sheng-quan,ZHOU Hong-zu,LIU Ta-si,YU Hui-min,YANG Da-jian,CHEN Xin-zi.Protective effect of water extracts of Ginseng on SH-SY5Y cell apoptosis induced by MPP+[J].Central South Pharmacy,2008,6(3):257-260. |
| |
| Authors: | HU Sheng-quan ZHOU Hong-zu LIU Ta-si YU Hui-min YANG Da-jian CHEN Xin-zi |
| |
| Affiliation: | HU Sheng-quan,ZHOU Hong-zu,LIU Ta-si,YU Hui-min,YANG Da-jian,CHEN Xin-zi(1.State Key Laboratory of Chinese Medicine and Molecular Pharmacology,Shenzhen Guangdong,518057;2.Hunan University of Traditional Chinese Medicine,Changsha 410007;3.Shenzhen Second Hospital,Shenzhen Guangdong 518035) |
| |
| Abstract: | Objective To investigate the protective effect of water extracts of Ginseng on SH-SY5Y cell apoptosis induced by MPP^＋ in culture.Methods MPP＋ induced SH-SY5Y cell apoptosis model was established.Water extracts of Ginseng was added into the culture to test its protective effect.The cell viability was measured by MTT.To test the morphology of SH-SY5Y,cells were observed by Hoechst 33258 staining and apoptotic rate was analyzed by FCM.Results The cell activity decreased remarkably induced by 0.5 mmol·L^-1 MPP^＋ for 60 h（P〈0.01）and the cells were positively stained with fluorochrome Hoechst 33258,presenting apoptotic rate to（40.52%±1.68%）,（P〈0.01）,However,water extracts of Ginseng obviously improved MTT value,and reduced cell apoptotic rate.Conclusion Water extracts of Ginseng have marked protective effect on SH-SY5Y cell apoptosis induced by MPP^＋. |
| |
| Keywords: | SH-SY5Y cells MPP^＋ WEG cell apoptosis |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
