| 大连湾牡蛎蛋白的分离提取及分子量测定 |
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| 引用本文： | 崔韶晖,王艳颖,崔杰,王岩,左明,宁洪良. 大连湾牡蛎蛋白的分离提取及分子量测定[J]. 大连民族学院学报, 2005, 7(1): 53-54,73 |
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| 作者姓名： | 崔韶晖 王艳颖 崔杰 王岩 左明 宁洪良 |
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| 作者单位： | 大连民族学院,生物工程系,辽宁,大连,116600;大连民族学院,生物工程系,辽宁,大连,116600;大连民族学院,生物工程系,辽宁,大连,116600;大连民族学院,生物工程系,辽宁,大连,116600;大连民族学院,生物工程系,辽宁,大连,116600;大连民族学院,生物工程系,辽宁,大连,116600 |
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| 基金项目： | 大连民族学院校科研和教改项目;大连民族学院校科研和教改项目 |
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| 摘 要： | 以大连湾牡蛎为实验材料．首先采用冰醋酸粗提法提取牡蛎蛋白，然后用葡聚糖凝胶(Sephadex-G100)进行柱层析分离，SDS—聚丙烯酰胺凝胶垂直板电泳分离蛋白，Kodak凝胶成像分析仪分析及测定分子量．结果显示大连湾牡蛎中含有分子量为55kd的牡蛎特征蛋门，同时分离出其它4种蛋白质，分子量介于33．0～63.4kd之间．
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| 关 键 词： | 牡蛎 蛋白质 Sephadex-G100柱层析 SDS-PAGE电泳 |
| 文章编号： | 1009-315X(2005)01-0053-02 |
The Extraction and Separation of the Protein from Ostrea Talienwhanensis Crosse and the Determination of the Molecular Weight |
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| CUI Shao-hui,WANG Yan-ying,CUI Jie,WANG Yan,ZUO Ming,NING Hong-liang. The Extraction and Separation of the Protein from Ostrea Talienwhanensis Crosse and the Determination of the Molecular Weight[J]. Journal of Dalian Nationalities University, 2005, 7(1): 53-54,73 |
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| Authors: | CUI Shao-hui WANG Yan-ying CUI Jie WANG Yan ZUO Ming NING Hong-liang |
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| Abstract: | Ostrea talienwhanensis crosse in Dalian Bay was taken as the experiment material. Protein was first extracted with acetic acid and was purified by Sephadex G-100 gel filtration chromatography, then the protein was separated by SDS-PAGE electrophoresis, and the molecular weight of the protein with Gel Image Station (Kodak) was determined. The results showed that oyster contained the protein of characterized of the oyster talienwhanenisis crosse 55kd and others 4 specie proteins whose molecular weight is between 33.0kd~63.4kd in ostrea talienwhanensis crosse. |
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| Keywords: | ostrea talienwhanensis crosse protein Sephadex G-100 chromatography SDS-PAGE electrophoresis |
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