HPLC法同时测定地黄喷雾剂中没食子酸和盐酸小糪碱含量

目的：建立地黄喷雾剂中没食子酸和盐酸小糪碱的HPLC含量测定方法。方法：采用ZORBAX SB-C18色谱柱（250 mm ×4.6 mm,5μm）,以乙腈-0.1%磷酸溶液为流动相,采用梯度洗脱程序,流速为1.0 ml·min^-1,检测波长为270 nm,柱温为30℃。结果：没食子酸和盐酸小檗碱的线性范围分别为20.36~305.40μg·ml-1（r=0.9996）和20.08~301.20μg· ml^-1（r=0.9998）,回收率分别为98.9%和98.5%,RSD分别为1.3%和1.5%。结论：该方法简单、可靠、准确,可用于地黄喷雾剂中没食子酸和盐酸小檗碱的含量测定。

Simultaneous Determination of Gallic Acid and Berberine Hydrochloride in Dihuang Sprays by HPLC

Objective：To establish an HPLC method for the determination of gallic acid and berberine hydrochloride in Dihuang sprays. Methods：A ZORBAX SB-C18 column （250 mm × 4. 6 mm,5 μm） was used, the mobile phase was acetonitrile-0. 1% phos-phoric acid with gradient elution. The flow rate was 1. 0 ml·min-1 , the column temperature was 30℃, and the detection wavelength wassetat270nm.Results：Thelinearrangeofgallicacidandberberinehydrochloridewas20.36-305.40 μg·ml-1（r=0.9996） and 20.08-301.20 μg·ml-1（r=0.999 8）, respectively. The average recovery was 98.9%（RSD =1.3%） and 98.5%（RSD =1. 5%） , respectively. Conclusion：The method is simple, reliable and accurate, which can be used in the determination of gallic acid and berberine hydrochloride of Dihuang sprays.