HPLC法测定氯沙坦钾氢氯噻嗪片中两组分的含量

目的：建立测定氯沙坦钾氢氯噻嗪片中氯沙坦钾、氢氯噻嗪含量的HPLC方法。方法：色谱柱为Zorbax Eclipse XDB-C8（200 mm ×4.6 mm,5μm）,流动相为0.01 mol·L^-1磷酸二氢钾溶液（用磷酸调节pH至3.0）-乙腈（70∶30）,流速为1.0 ml· min^-1,柱温为35℃,检测波长为271 nm,进样量为20μl。结果：氯沙坦钾的质量浓度在39.63~118.89μg · ml^-1（ r =0.9999）,氢氯噻嗪的质量浓度在9.47~28.41μg·m^l-1（r=0.9996）范围内与其峰面积呈良好的线性关系；平均回收率分别为99.4%（RSD=1.0%,n=9）,99.8%（RSD=0.9%,n=9）。结论：该方法简便、快速、准确,重复性好,适用于氯沙坦钾氢氯噻嗪片中两组分的含量测定。

Determination of two groups in Losartan Potassium and Hydrochlorothiazide Tablets by HPLC

Objective：To establish an HPLC method for the determination of two ingredients in losartan potassium and hydrochlo-rothiazide tablets. Methods： Losartan potassium and hydrochlorothiazide were separated on a Zorbax Eclipse XDB-C8 （ 200 mm × 4. 6 mm, 5 μm）column, the mobile phase consisted of 0. 01 mol·L^-1 monopotassium phosphate solution（adjusting pH to 3. 0 with phosphoric acid）-acetonitrile（70∶30）with the flow rate of 1. 0 ml·min^-1. The column temperature was set at 35℃, the detection wavelength was 271nm and the injection volume size was 20 μl. Results：The linear range of losartan potassium and hydrochlorothiaz-ide was 39.63-118.89 μg·ml^-1（r=0.999 9） and 9.47-28.41 μg·ml-1（r=0.999 6） with the average recovery of 99.4%（RSD=1. 0%,n=9）and 99. 8%（RSD=0. 9%, n=9）, respectively. Conclusion： The method is simple, fast, accurate and reproduci-ble, which is suitable for the determination of losartan potassium and hydrochlorothiazide tablets.