新型聚乙烯亚胺衍生物在COS-7 细胞中转染和毒性的研究

目的:研究以乙二醛为连接剂的聚乙烯亚胺(Polyethyleneimine,PEI)衍生物Polyimine-PEI对非洲绿猴肾癌细胞COS-7的转染活性和细胞毒性的影响。方法:以荧光素酶质粒为报告基因,研究高分子与DNA的复合物在COS-7细胞的转染活性,用MTT方法研究高分子对COS-7细胞的毒性。结果:COS-7细胞实验显示,Polyimine-PEI具有很低细胞毒性,其毒性显著低于PEI25kDa,同时也具有高效输送质粒的能力。结论:Polyimine-PEI是一种新型的高效,低毒在基因治疗领域有相当前景的非病毒载体。

Transfection and Cytotoxicity Studies of Novel Polyethyleneimine Derivative in COS-7 cells

Objective:To investigate the transfection efficiency and cell cytotoxicity of Polyethyleneimine(PEI) derivative linking with glyoxal.Methods:Luciferase plasmid was used as the reporter gene to investigate transfection efficiency in COS-7 cells.Cytotoxicity was detected by MTT method.Results:COS-7 cells transfection and cytotoxicity results suggested that the gene carrier(Polyimine-PEI) was less cytotoxicity than that of PEI25kDa,and expressed equal transfection efficiency with PEI25kDa and Lipofectamine 200...