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| 三七中分离微生物对其转化的初步研究 | |
| 引用本文： | 赵方允,虞泓,陈自宏,曾文波,殷勤红,葛锋. 三七中分离微生物对其转化的初步研究[J]. 医学研究杂志, 2011, 40(8): 47-50 |
| 作者姓名： | 赵方允 虞泓 陈自宏 曾文波 殷勤红 葛锋 |
| 作者单位： | 1. 650091,昆明,云南大学中草药生物资源研究所云百草实验室;650051,昆明市延安医院 2. 云南大学中草药生物资源研究所云百草实验室,昆明,650091 3. 昆明理工大学生命科学与技术学院,650224 |
| 基金项目： | 国家自然科学基金资助项目 |
| 摘 要： | 目的从云南三七材料中分离共生菌,对三七进行发酵转化,研究转化后皂苷成分的变化。方法采用平板划线分离法,从三七根茎、花、种子等材料中分离纯化共生菌,经液体发酵,通过TLC、UV、HPLC等检测方法,对转化前后皂苷变化进行分析。结果共分离出真菌27株,细菌2株初步筛选出两株对皂苷单体转化明显的菌株,转化后总皂苷含量有所增加。结论从三七材料中分离的真菌可以对三七进行定向转化,得到稀有皂苷,并提高总皂苷含量。 |
| 关 键 词： | 三七 真菌 发酵 人参皂苷 皂苷 |
| 收稿时间： | 2010-12-14 |
| 修稿时间： | 2011-05-31 |
A Study on Transformation of Panax Notoginseng by Microbes Isolated from Notoginseng |
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| Zhao Fangyun,Yu Hong,Chen Zihong,Zeng Wenbo,Yin Qinhong,Ge Feng. A Study on Transformation of Panax Notoginseng by Microbes Isolated from Notoginseng[J]. Journal of Medical Research, 2011, 40(8): 47-50 | |
| Authors: | Zhao Fangyun Yu Hong Chen Zihong Zeng Wenbo Yin Qinhong Ge Feng |
| Affiliation: | Zhao Fangyun,Yu Hong,Chen Zihong,Zeng Wenbo,Yin Qinhong,Ge Feng.Yunnan Herbal Laboratory,Institute of Herb Biotic Resources,Yunnan University,Yunnan 650091,China |
| Abstract: | Objective The microbes were isolated from notoginseng materials sampled in Yunnan.After the microbes were isolated into transforming notoginseng,the chemical ingredients of saponin were determined.Methods With plate streaking method,the microbes were isolated from samples of notoginseng roots,flowers,and seeds.After the microbes being isolated into liquid fermentation of the notoginseng samples,the changes of saponins were analysed by TLC,UV and HPLC methods.Results The twenty-seven microbes were identified... |
| Keywords: | Panax notoginseng Fungi Fermentation Ginsenosides Saponin |
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