苦参对感染柯萨奇B3病毒乳鼠搏动心肌细胞的保护 …

目的 研究苦参中对抗柯萨奇Ｂ３病毒（ＣＶＢ３）的有效成份，即抗柯萨奇注射液（ＳＦＡ）抗ＣＶＢ３病毒的作用。方法 将心肌细胞分为４组，感染组（ｎ＝８），只感染病毒，不加ＳＦＡ；治疗组（ｎ＝８）；感染病毒后加入ＳＦＡ（１００ｍｇ／Ｌ），药物组（ｎ＝６）；不感染病毒，只加入ＳＦＡ（１００ｍｇ／Ｌ）；对照组（ｎ＝６）；不感染病毒，不加ＳＦＡ。结果 在感染病毒后第２、３、５ｄ，感染组心肌细胞的病变程度较其余

Effect of sophora flavescens Ait on cultured beating myocardial cells of coxsackie B3 virus infected newborn rat

OBJECTIVE: Coxsackie B viruses (CVB3) are considered to be the most common etiologic agents of viral myocarditis. There are not any special anti-CVB3 drugs yet. From previous studies, the anti-CVB3 affect of sophora flavescens ait (SFA) had been discovered. Our experiment was to study the anti-CVB3 ability of SFA to cultured heating myocardial cells of CVB3 infected newborn rat. METHODS: The myocardial cells were divided into four groups: 1. infected group (n = 8), infected only with CVB3, not adding SFA; 2. treated group (n =8), infected with CVB3, adding SFA (100 microg/ml); 3. drug group (n=6), adding SFA (100 microg/ml) only; 4. control group (n = 6), not infected with CVB3, not adding SFA. RESULTS: The myocardial cells of the infected group had cell pathogenic effect (CPE) on the second day after virus inoculation, the CPE progressed rapidly from + to ++++. In contrast, no CPE in the other three groups was found. The LDH and SGOT of the infected group were higher than that in the other three groups, showing a significant difference (P <0.05). The virus titer of the infected group was higher than that of the treated group. There was no influence on normal myocardial cells if the concentration of SFA was lower than 300 microg/ml. When the concentration of SFA was 6.25 microg/ml 200 microg/ml, it showed protective effect on infected myocardial cells. CONCLUSIONS: The results of the experiment suggest that SFA might inhibit CVB3 replication in myocardial cells.