人参皂苷的仿生提取研究

通过体外模拟胃肠道环境,建立一种提取人参皂苷的仿生方法.考察了提取条件对配制的仿生胃液和仿生肠液提取人参皂苷浓度的影响.基于高效液相色谱-三重四极杆质谱的多反应监测模式建立定量分析Re,Rg1,20(S)-Rf,Rb1,Ro,Rc,Rb2,Rd等8种人参皂苷的方法,并比较了仿生和超声两种提取方法的人参皂苷提取效率.结果...

Research on biomimetic extraction of ginsenoside

A biomimetic method for the efficient extraction of ginsenoside was developed via the in vitro simulation of gastrointestinal tract environment.Two kinds of solvent,biomimetic gastric juice and biomimetic intestinal juice,were prepared to extract ginsenosides from ginseng.The extract conditions were investigated and optimized in detail.A quantitative method based on high performance liquid chromatography-triple quadrupole mass spectrometer in multiple reaction monitoring mode was established to detect the concentrations of extracted ginsenoside Re,Rg1,20(S)-Rf,Rb1,Ro,Rc,Rb2 and Rd.The extraction efficiency of the developed biomimetic method was further compared with that of an optimized ultrasonic extraction method.The quantitative results showed that the extraction concentration of ginsenosides in gastric juice was slightly higher than that in intestinal juice.The optimized conditions were as follows:refluxing at 37.0℃for 60 min with a ratio of liquid to solid of 15:1.The biomimetic method was validated and showed good repeatability,precision,and accuracy,and could extract ginsenoside from ginseng effectively.Under the optimized conditions,the concentration of ginsenosides extracted by biomimetic method was 10.4%-56.9%higher than that by ultrasonic method.Biomimetic method doesn’t require organic solvent and is suitable for the rapid extraction of ginsenoside.