PNIPAAm-g-EVA/明胶支架双层人工皮肤的细胞相容性研究

构建了PNIPAAm-g-EVA/明胶支架复合人工皮肤样品,并初步考察了该人工皮肤的细胞相容性。由于明胶支架的生物相容性已经得到充分验证,主要选择成纤维细胞为实验细胞,PNIPAAm-g-EVA膜为研究对象。采用含有胶原酶的消化液消化组织块,在较短时间内可获得足够数量的成纤维细胞。成纤维细胞与PNIPAAm-g-EVA膜共培养结果表明,成纤维细胞在膜表面粘附力强,生长、增殖情况良好。说明与PNIPAAm-g-EVA膜共培养并未影响细胞的正常生理功能,PNIPAAm-g-EVA具有良好的细胞相容性。

Cell biocompatibility of PNIPAAm-g-EVA/gelatin bilayer artificial skin

In this study a gelatin/PNIPAAm-g-EVA bilayer artificial skin had been constructed and its cell compatibility had also been assessed.As the good biocompatibility of gel had already been proved.So we focus on assessing the cell compatibility of PNIPAAm-g-EVA membrane.Rat skin fibroblasts were cultured in vitro with PNIPAAm-g-EVA membrane for different periods,and the results indicated that the proliferation of fibroblasts on PNIPAAm-g-EVA membrane increased with time significantly.Imnunohistochemical staining of fibroblasts were positive.Therefore,it can be concluded that the PNIPAAm-g-EVA membrane has good cytocompatibility on the basis of positive effect on the attachment,proliferation and growth of fibroblasts.