复方红景天制剂对高正加速度应激大鼠血清皮质酮及心肌组织糖皮质激素受体的影响

目的研究复方红景天制剂对高正加速度(positive acceleration,+Gz)应激大鼠血清皮质酮及心肌组织糖皮质激素受体的影响。方法 72只雄性SD大鼠随机分为6组,分别为空白对照组、应激对照组、+10Gz应激组以及药物低、中、高剂量组,每组12只。各组均灌胃给药14 d,低、中、高剂量组大鼠每天分别按0.75g/kg、1.5 g/kg和3.0 g/kg给予复方红景天中药制剂,其余3组动物给等量的生理盐水。第15天进行+10 Gz离心机暴露,ELISA方法测定各组动物血清皮质酮浓度,免疫印迹法分析各组动物心肌组织糖皮质激素受体表达变化。结果与空白对照组和应激对照组相比,+10 Gz应激组大鼠血清皮质酮浓度明显升高,而心肌组织糖皮质激素受体表达水平明显降低(P0.01,P0.05)。复方红景天制剂预处理可以降低+10 Gz应激大鼠血清皮质酮浓度,上调心肌组织糖皮质激素受体表达水平,药物中、高剂量组大鼠血清皮质酮浓度明显低于+10 Gz应激组(P0.05),而心肌组织糖皮质激素受体表达水平明显高于+10 Gz应激组(P0.01)。结论复方红景天制剂可以调节+Gz应激大鼠血清糖皮质激素浓度及其心肌受体表达水平,可能与该制剂对持续正加速度致心肌损伤的防护作用有关。

Effects of rhodiola crenulata compound on serum corticosterone and myocardial glucocorticoid receptor in rats exposed high sustained positive acceleration

Objective To study the effect of rhodiola crenulata compound on serum corticosterone and myocardial glucocorticoid receptor （ GR） in rats exposed high sustained ＋Gz.Methods Seventy-two healthy SD rats were randomly divided into six groups： blank control group, stress control group, ＋10 Gz stress group and low, medium, high dose drug group, with twelve rats in each.20 mL/kg menstruum was fed to each rat once per day for 14 days.Low, medium and high dose drug group were fed with rhodiola crenulata compound at doses of 0.75 g/kg, 1.5 g/kg and 3.0 g/kg respectively, the other three groups were fed with equal volume saline.Rats were exposed to high ＋Gz in 15th day.The concentration of corticosterone in the serum of each group rats was detected with ELISA. Western blot was used to detect the expressions of GR in the myocardium of each group rats .Results The content of corticosterone was significantly elevated in ＋10Gz stress group, while the expression of GR in the myocardium was markedly declined （P〈0.01,P〈0.05）.However, compound preconditioning could decrease concentration of＆amp;nbsp;corticosterone in the serum and enhance the expression of GR in the myocardium from rats after ＋10 Gz exposure. The corticosterone concentration of medium and high dose drug groups was significantly lower and the level of GR expression in the myocardium was significantly higher than that of ＋10 Gz stress group （ P〈0.05 , P〈0.01 ） . Conclusion Rhodiola crenulata compound preconditioning could regulate the concentration of corticosterone in the serum and the level of GR expression in the myocardium of the rats exposed ＋10 Gz, which may be related with its protective effect on high sustained ＋Gz-induced injury of myocardium.