舒芬太尼后处理对大鼠肠缺血再灌注时肺组织损伤的影响 |
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引用本文: | 郑巧芬,曹定睿,赵丽,支晓雁,李兴艳. 舒芬太尼后处理对大鼠肠缺血再灌注时肺组织损伤的影响[J]. 中国医疗前沿, 2010, 5(20): 21-22 |
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作者姓名: | 郑巧芬 曹定睿 赵丽 支晓雁 李兴艳 |
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作者单位: | 山西医科大学第一附属医院麻醉科,太原,030001 |
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摘 要: | 目的探讨舒芬太尼后处理对大鼠肠缺血再灌注时肺组织损伤的影响。方法将40只成年雄性Wistar大鼠,随机分为5组(n=8):假手术组(S组)、肠缺血再灌注组(I/R组)及舒芬太尼1μg/kg、5μg/kg、10μg/kg后处理组(SP1-3组)。采用放射免疫法测定血清及肺组织中肿瘤坏死因子(TNF-α)的含量;免疫组织化学法测定肺组织中NF-κB表达水平。结果与S组相比,其余各组TNF-α含量及NF-κB表达水平升高(P〈0.05);与I/R组相比,SP1-3组TNF-α含量及NF-κB表达水平降低(P〈0.05);SP1-3组间上述指标比较差异无统计学意义(P〉0.05)。结论舒芬太尼后处理减轻大鼠肠缺血再灌注时肺损伤的机制可能与下调NF-κB的表达、降低肺组织炎性反应有关。
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关 键 词: | 舒芬太尼 肺损伤 缺血再灌注损伤 肿瘤坏死因子α 核因子κB |
Effects of Sufentanil post-conditioning on acute lung injury induced ischemia-reperfusion in rats |
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Affiliation: | ZHENG Qiao-fen,CAO Ding-rui,ZHAO Li,et al.Department of Anesthesiology,First Affiliated Hospital,Shanxi Medical University,Taiyuan 030001,China |
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Abstract: | Objective To investigate the effects of Sufentanil post-conditioning(SP) acute lung injury induced by ischemiareperfusion(I/R) in rats.Methods Forty Wistar rats were randomly divided into 5 groups(n=8):Sham-operation group(S),I/R group(I/R) and 1μg/kg、5μg/kg、10μg/kg Sufentanil post-conditioning group(SP1-3).TNF-α in the serum and lung tissue was determined using radio-immunifaction method.Expression of NF-κB P65 in the lung tissue was determined by immuno-histochemical staining.Results Compared with the sham-operated group,serum and lung lever TNF-α was increased significantly in the other groups(P0.05),and NF-κB p65 protein in the lung was increased(P0.05).Compared with the I/R group,TNF-α in the serum and lung tissues was decreased significantly following Sufentanil treatment(P0.05),and the expression of lung NF-κB was markedly ameliorated(P0.05).There was no significant different in the indices mentioned above among group SP1-3.Conclusion Sufentanil post-conditioning can ameliorate the lung injury induced by intestine I/R throμgh inhibition of NF-κB activation and inflammatory response of lung tissue. |
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Keywords: | Sufentanil Lung injury Ischemia-reperfusion injury TNF-α NF-κB |
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