nm23-H1基因表达与胃癌生物学行为的关系

目的 :探讨nm2 3 H1基因表达与胃癌生物学行为的关系。方法 :应用原位杂交 (ISH)及免疫组织化学技术 ,检测nm2 3 H1在 2 7例胃癌及相应的胃正常粘膜组织中mRNA和蛋白的表达。结果 :nm2 3 H1mRNA及蛋白在胃癌及相应的正常组织中的表达无显著性差异 (P >0 .0 5 )。在有淋巴结转移的胃癌中nm2 3 H1mRNA表达显著低于无淋巴结转移组(P <0 .0 1) ;nm2 3 H1蛋白在有淋巴结转移的胃癌中表达阳性率为 2 6 .8% ,显著低于在无淋巴结转移的胃癌中表达阳性率83 % (P <0 .0 5 )。nm2 3 H1mRNA及蛋白在有浆膜浸润 (T3、T4 )胃癌中的表达显著低于无浆膜浸润 (T1、T2 )的胃癌 (P <0 .0 5 )。结论 :nm2 3 H1基因表达与胃癌浸润及淋巴结转移有关

The Relationship Between nm23-H1 Gene Expression and Biological Behavior of Gastric Cancer

Objective The experiment was designed to evaluate the relationship between nm23?H1 gene expression and biological behavior of gastric cancer.Methods The expression of nm23?H1 gene was observed in 27 cases of gastric cancer and matching normal tissues using ISH and immunohistochemistry technigue. Results There was no significant difference in expression of nm23?H1 mRNA and protein between gastric cancer and matching normal tissue( P >0.05). nm23?H1 mRNA expression was significantly reduced in the group of gastric cancer with lymph nodes(LN) metastasis. The positive rate of nm23?H1 protein expression was 26.8% in the group of gastric cancer with LN metastasis, 83% in the group of gastric cancer without LN metastasis,respectively( P <0.05). nm23?H1 mRNA and protein expression was significantly reduced in gastric cancer with infiltrated serosa(T 3/T 4) than that in the group without infiltrated serosa.Conclusions nm23?H1 gene expression correlates with infiltration and lymph node metastasis of gastric cancer.