| 前列腺癌基因诊断芯片的临床应用 |
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| 引用本文： | 蔡岳斌,何慧婵,刘良式,刘文华,江福能,魏鸿蔼,钟惟德,. 前列腺癌基因诊断芯片的临床应用[J]. 中国医学工程, 2005, 13(6): 597-599 |
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| 作者姓名： | 蔡岳斌 何慧婵 刘良式 刘文华 江福能 魏鸿蔼 钟惟德 |
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| 作者单位： | 1. 广州医学院附属广州市第一人民医院,广东,广州,510180
2. 中山大学生命科学院,广东,中山,510275 |
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| 摘 要： | 目的应用基因芯片诊断前列腺癌.方法提取前列腺癌及正常前列腺组织总DNA并纯化mRNA,以包含了9个前列腺癌相关的特异基因和1个参照基因的xy检测系统cDNA临床芯片,对前列腺癌及正常前列腺组织的基因表达谱进行分析.结果9个前列腺癌相关基因检测中癌与正常组织存在显著差异,其中显著上调的有7条;显著下调有2条.结论前列腺癌临床基因诊断芯片作为前列腺癌分子水平的诊断的方法,有望提高前列腺癌的检出率.
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| 关 键 词： | 前列腺肿瘤/癌 基因 诊断 DNA芯片 |
| 文章编号： | 1672-2019（2005）06-0597-03 |
| 收稿时间： | 2005-10-25 |
| 修稿时间： | 2005-10-25 |
Clinical application of cDNA chip on prostate cancer diagnosis |
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| CAI Yue-bin,HE Hui-chan,LIU Liang-shi,LIU Wen-hua,JIANG Fu-neng,WEI Hong-ai,ZHONG Wei-de. Clinical application of cDNA chip on prostate cancer diagnosis[J]. China Medical Engineering, 2005, 13(6): 597-599 |
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| Authors: | CAI Yue-bin HE Hui-chan LIU Liang-shi LIU Wen-hua JIANG Fu-neng WEI Hong-ai ZHONG Wei-de |
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| Abstract: | [objective] To diagnose prostatic cancer (CaP) with cDNA chip. [Methods] Total RNA was isolated from prostate cancer and from normal prostate, and poly(A) RNA was further purified. Then it was analysed for differentially expressed genes in CaP and normal prostate by cDNA chip with 9 prostate-associated specific genes and 1 control gene under XY detection system. [Results] There were differentially expressions in the nine prostate-associated specific genes in CaP as compared with normal prostate, in which, 7 were significantly upregulated and 2 were down-regulated. [Conclusion] As an diagnostic approach at molecular level, the cDNA chip is supposed to elevate the detection rate of CaP. |
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| Keywords: | prostatic neoplas carcinoma genes DNA chip |
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