三种抗氧化剂对青石棉诱发微核,多核及转化细胞生长的影响

在青石棉诱发Ｖ７９细胞微核与多核模型的基础上，以维生素Ｅ（ＶＥ），β－胡萝卜素（β－Ｃａｒ）或亚硒酸钠（Ｓｅ）分别与青石棉同时处理细胞，观察上述抗氧化剂对其微核细胞率与多核细胞率的影响．发现ＶＥ和Ｓｅ可分别降低其微核细胞率或多核细胞率，β－Ｃａｒ对二者均无影响．采用青石棉诱发的ＢＡＬＢ／ｃ－３Ｔ３恶性转化细胞，观察上述物质对其克隆形成率（ＣＦＥ）和细胞生长的影响．发现Ｓｅ，β－Ｃａｒ和ＶＥ分别在１０，６和２５０μｍｏｌＬ－１以上浓度出现ＣＦＥ降低，且细胞生长受抑．

Effects of three antioxidants on crocidolite induced micronuclei and multinuclei and growth of transformed cells

Based on the model of induction of micronuclei and multinuclei by crocidolite in V79 cell line, the cells were treated with vitamin E (VE, 6.2-166.7 μmol·L^-1), β-carotene(β-Car, 0.5-4.0 μmol·L^-1), or sodium selenite (Se, 0.01-0.4 μmol·L^-1), respectively, in combinations with crocidolite(0.62 μg·cm^-2) for 24 h. The results indicated that VE had an inhibitory effect on the incidences of micronucleated cells, that Se reduced that of multinucleated cells in a narrow range of concentrations(0.04-0.08 μmol·L^-1), and that β-Car had no effect on that of either micronucleated or multinucleated cells. Transformed BALB/c-3T3 cells induced by crocidolite were used for the following study: the cells were treated with a series of doses of Se(10, 20 μmol·L^-1), β-Car(6, 12 μmol·L^-1), or VE(250, 500 μmol·L^-1), respectively, then the growth and colony formation efficiency of the cells were observed in different times (42-168 h). It was found that at the concentrations above 10, 6, or 250 μmol·L^-1, respectively, Se, β-Car, or VE inhibited the growth and colony formation of the transformed cells. Of the three antioxidants, Se and β-Car showed the above effects near their physiological concentrations in blood, but VE did not unless up to more than 10 folds of its physiological concentration.