不同妊娠时期人胎盘绒毛细胞连接蛋白基因表达的研究

目的 探索妊娠不同时期人胎盘绒毛细胞连接蛋白基因表达的规律及其与细胞分化的关系。方法 采用多种细胞连接蛋白基因ｃＤＮＡ探针，通过Ｎｏｒｔｈｅｒｎ印迹法，研究了不同妊娠时期胎盘绒毛细胞内Ｃｘ基因的表达状态。结果 １．Ｃｘ基因表达有器官特异性，绒毛细胞中Ｃｘ４３、Ｃｘ３２及Ｃｘ４０表达，而Ｃｘ３１．１、Ｃｘ３３、Ｃｘ３７、Ｃｘ４６不表达；２．在不同发育阶段的胎盘阶段的胎盘绒毛中，Ｃｘ４３、Ｃｘ３２、Ｃ

CONNEXIN GENES EXPRESSION OF HUMAN PLACENTA AT DIFFERENT PREGNANT STAGES

Objective\ To investigate the role of connexin gene expression of placental chorial cells and its relationship with cellular differenciation. Methods\ Northern blot method with cDNA molecular probes of connexin genes Cx31\^1,Cx32,Cx33,Cx37,Cx40,Cx43,Cx46 were used in the experiment. Results\ Northern blot demonstrated that:1.The expression of Cx gene had organic specificity.In human placental chorion, Cx43,Cx40 and Cx32 were expressed while other Cx genes weren't 2.The expression of Cx genes showed different levels in different stages of pregnancy.3.The expression levels of Cx genes were compatible with the differenciation and development of placental villous cells. Conclusion\ Cx gene expression appears more likely to play a key role in regulating differentiation, proliferation of chorial cells and placental morphogenesis.