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MeJA熏蒸叶片对新疆沙冬青根细胞跨膜离子流及抗盐性的影响
引用本文:刘蕾,高海波,沈应柏.MeJA熏蒸叶片对新疆沙冬青根细胞跨膜离子流及抗盐性的影响[J].植物生理学通讯,2014(4):477-482.
作者姓名:刘蕾  高海波  沈应柏
作者单位:[1]北京林业大学 生物科学与技术学院,北京100083 [2]北京林业大学 林木育种国家工程实验室,北京100083 [3]临沂大学生命科学学院,山东临沂276005
基金项目:国家自然科学基金(31270655和30871727)、国家863计划(2011AA100200)和高等学校博士学科点专项科研基金(20090014110014).
摘    要:土壤盐渍化是植物生长的主要逆境之一。茉莉酸类物质与植物抗逆性密切相关。以国家二级保护植物新疆沙冬青为实验材料,利用非损伤微测技术,从动态离子流转运的角度研究了MeJA预处理后Nacl处理对新疆沙冬青根部K+、H+、Na+流的影响。结果表明,MeJA预处理的新疆沙冬青经盐胁迫后根冠区瞬时K+外流低于未经MeJA预处理的对照植株,H+外流强度大于对照植株;新疆沙冬青根尖区域(0~3000μm)经MeJA和Nacl(50mmol·L-1,24h)预处理,解除NaCl胁迫后观察到Na+外流和H+内流,流速高于对照植株。表明适当浓度MeJA预处理能有效提高新疆沙冬青苗期抗盐性。

关 键 词:盐胁迫  H+-ATPase  MeJA  非损伤微测技术

Effects of MeJA Fumigation to Leaves on Ion Flux in Apical Region of Ammopiptanthus nanus and Salt Resistance
LIU Lei,GAO Hai-Bo,SHEN Ying-Bai.Effects of MeJA Fumigation to Leaves on Ion Flux in Apical Region of Ammopiptanthus nanus and Salt Resistance[J].Plant Physiology Communications,2014(4):477-482.
Authors:LIU Lei  GAO Hai-Bo  SHEN Ying-Bai
Affiliation:1 College of Bzologlcal Sciences and Technology, National Engineering Laboratory for Tree Breeding, Bezjzng Forestry University, Beijing 100083, China; 3College of Life Sciences, Linyi University, Linyi, Shandong 276005, China)
Abstract:Soil salinization is one of serious factors restricting the expansion of agriculture and forestry around the world. Jasmonic acid is closely associated with plants on salt tolerance. Ion fluxes were studied in the roots of Arnmopiptanthus nanus, a second class national-level protected plant, by using non-invasive micro-test technique. The result showed that the plants with MeJA pretreatment have lower efflux of K+ and higher efflux of H+ than the plants without pretreatment under the stress of NaC1 in the root cap. After short-term salt stress (50 mmol.L-1NaC1, 24 h), the plants with MeJA pretreatment have higher Na+ efflux and H+ influx than the plants without pretreatment in root apex. The results indicate that suitable concentration of MeJA pretreatment can increase salt stress resistance ofA. nanus at seedling stage.
Keywords:salt stress  H+-ATPase  MeJA  non-invasive micro-test technique
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