干扰素诱导跨膜蛋白3 rs12252多态性与乙型流感病毒感染相关性研究

目的 了解干扰素诱导跨膜蛋白3(IFITM3) rs12252多态性与乙型流感感染之间的关联.方法 选择2014年1月-2015年4月北京地区流感样病例(ILI)监测乙型流感感染病例80例、严重急性呼吸道感染病例(SARI)监测乙型流感感染病例85例和健康对照人群65例.提取基因组DNA,使用高分辨率熔解曲线法和Sanger测序法检测IFITM3rs12252多态性..结果 ILI病例组IF-ITM3 rs12252基因型分布频率与对照组无显著性差异,但SARI病例组与对照组相比基因型分布频率具有显著差别.61.2％的SARI病例为IFITM3 rs12252 CC基因型,明显高于ILI病例和对照人群.隐性遗传模型显示CC基因型个体比TT基因型和CT基因型个体有更高的风险发展为乙型流感重症感染(OR =4.73,95％CI 2.42 ～9.22).结论 IFITM3 rs12252 CC基因型会导致较高的乙型流感重症感染风险.

The Association between IFITM3 rs12252 Polymorphism and Influenza B Virus Infection

Objective To investigate the genetic polymorphism oflFITM3 rs12252 in influenza-like illness cases and severe acute respiratory infection cases in Beijing area from 2014 to 2015.Methods The genomic DNA was extracted from swab samples.Then the phylogeny of IFITM3 rs12252 was determined by high resolution melt (HRM) analysis and Sanger sequencing.Results No significant difference of IFITM3 rs12252 distribution was observed between controls and ILI cases.However,61.2％ SARI cases showed CC genotype,which was significant higher than that of controls.Recessive genetic model suggested that the attributable risk(OR) of severe influenza B virus infection in cases with CC genotype was 4.73 higher than those cases with CT or TT genotype (95％ CI 2.42 ～ 9.22).Conclusions Influenza B virus-infected cases with CC genotype was more likely to result insevere infection.