Flow‐Based Enzymatic Ligation by Sortase A |
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Authors: | Rocco L. Policarpo Hansol Kang Dr. Xiaoli Liao Amy E. Rabideau Mark D. Simon Prof. Bradley L. Pentelute |
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Affiliation: | Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 (USA) |
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Abstract: | Sortase‐mediated ligation (sortagging) is a versatile, powerful strategy for protein modification. Because the sortase reaction reaches equilibrium, a large excess of polyglycine nucleophile is often employed to drive the reaction forward and suppress sortase‐mediated side reactions. A flow‐based sortagging platform employing immobilized sortase A within a microreactor was developed that permits efficient sortagging at low nucleophile concentrations. The platform was tested with several reaction partners and used to generate a protein bioconjugate inaccessible by solution‐phase batch sortagging. |
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Keywords: | flow chemistry immobilization microreactor protein modification sortase A |
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