丹参冠瘿组织高产株系选择和丹参酮的产生

丹参(Salvia miltiorrhiza)是治疗心血管系统疾病的重要中草药。前文我们已报道了丹参冠瘿组织培养的部分研究结果^[1].利用根癌农杆菌(Agrobacterium tumefaciens)Ti 质粒转化产生的冠瘿组织进行高产株系选择的研究还很少，本文报道丹参冠瘿组织高产株系选择和丹参酮产生的研究结果。

Selection of a High Tanshinone producing Crown Gall Strain and Production of Tanshinone in the Strain

Crown galls were induced by direct infection of sterile seedlings of Salvia miltiorrhiza with Agrobacterium tumefaciens C58 and subcultured in 67 V hormone free medium by successively selecting red cell aggregates. A high tanshinone producing crown gall strain C1 was obtained after 12 months in subculture. It grows well and keeps high tanshinone producing characteristic in liquid stationary culture. It was obvious that yeast extract and Armillaria mellea as elicitors could promote the strain C1 to produce tanshinone. Tanshinone content of the strain C1 cultures was over three times higher than that of the crude drugs. The results indicated that the crown gall tissue and elicitor technique could be provided some new clues for producing tanshinone under the condition of liquid stationary culture.