| 运用FQ RT-PCR和Western blot检测Claudin-1在人类大肠癌细胞株的表达 |
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| 引用本文： | 陈琳,姜泊,张亚历,张宏权,龚伟,卢青. 运用FQ RT-PCR和Western blot检测Claudin-1在人类大肠癌细胞株的表达[J]. 广西医科大学学报, 2007, 24(2): 205-207 |
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| 作者姓名： | 陈琳 姜泊 张亚历 张宏权 龚伟 卢青 |
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| 作者单位： | 1. 南方医科大学南方医院消化科,广州,510515
2. 生命与营养科学研究所,卡罗林斯卡学院 SE-14157,呼丁格市 瑞典 |
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| 摘 要： | 目的:探讨密连接蛋白Claudin-1与人类大肠癌进展的关系.方法:采用实时荧光定量RT-PCR(FQ RT-PCR)和Western blot,检测Claudin-1在3株不同分期人类大肠癌细胞株中的表达.结果:Claudin-1在大肠癌Dukes'type C期细胞株SW620强表达,Dukes'type B期细胞株SW480次之,Dukes'type A 期细胞株SW1116最弱.结论:Claudin-1在人类大肠癌细胞株中有表达,其表达量与大肠癌分期有关.
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| 关 键 词： | 大肠癌 Claudin-1 实时荧光定量RT-PCR Western blot |
| 修稿时间： | 2006-11-22 |
STUDY THE EXPRESSION OF CLAUDIN-1 IN HUMAN COLORECTAL CARCINOMA CELL LINES BY FQ RT-PCR AND WESTERN BLOT |
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| Chen Lin,Jiang Bo, Zhang Yali,et al.. STUDY THE EXPRESSION OF CLAUDIN-1 IN HUMAN COLORECTAL CARCINOMA CELL LINES BY FQ RT-PCR AND WESTERN BLOT[J]. Journal of Guangxi Medical University, 2007, 24(2): 205-207 |
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| Authors: | Chen Lin Jiang Bo Zhang Yali et al. |
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| Affiliation: | Institute for Digestive Diseases, Nan Fang Hospital, Nan Fang Medi- cal University, Guangzhou 510515 China |
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| Abstract: | |
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| Keywords: | colorectal carcinoma Claudin-1 FQ RT-PCR Western blot |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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