血管平滑肌细胞凋亡在腹主动脉瘤发病中的作用

目的：探讨腹主动脉瘤（AAA）中膜血管平滑肌细胞（VSMC）密度降低的机制。方法：选取人体肾下AAA及正常腹主动脉组织（NA）标本，采用免疫组化及原位末端DNA标记技术，测定中膜VSMC，凋亡细胞及其相关蛋白，计算机图像分析并计算VSMC密度及凋亡指数。结果：与NA相比，AAA中膜VSMC密度降低，VSMC凋亡指数及其相关蛋白P53，P21明显增加，而bcl-2无显变化。结论：VSMC凋亡在细胞水平参与腹主动脉结构损伤与重构，促进AAA形成。

Vascular smooth muscle cells apoptosis in human abdominal aortic aneurysm

Objective: To explore the effect of vascular smooth muscle cells (VSMC) apoptosis on abdominal aortic aneurysms(AAA).Methods: Tissue samples of human infrarenal aneurysmal and normal aorta were evaluated.In situ end-labeling of DNA fragments(TUNAL) and immunohistochemistry determined VSMC,apoptosis cells and apoptosis markers.VSMC density and apoptosis index were counted with the use of computer-generated image analysis.Results: In comparison to normal aorta,VSMC density in AAA was decreased, and VSMC apoptosis and apoptosis markers,P53 or P21,were increased obviously.But significant difference of bcl-2 could not be demonstrated when compared with the controls.Conclusion: VSMC apoptosis is connected with abdominal aortic structure damage and remodeling in the cellular level,which contributes to AAA formation.