A procedure for the extraction of chloroplast DNA from broad-leaved tree species |
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| Authors: | H Michaud R Lumaret J P Ripoll L Toumi |
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| Affiliation: | (1) Département de Biologie des Populations, Centre d'Ecologie Fonctionnelle et Evolutive (CEFE) Louis Emberger, CNRS, B.P. 5051, 34033 Montpellier Cedex, France |
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| Abstract: | A method for the extraction of ctDNA from isolated chloroplast was developed. This method is simple and adapted particularly
to broad-leaved trees, including sclerophyllous species with high phenolic and polysaccharide contents. This method includes
two major steps: first, chloroplasts are isolated in non-aqueous solutions to avoid oxidation and phenolic problems; second,
ctDNA is extracted from the chloroplasts using aqueous solutions and specific methods to provide highly purified ctDNA. |
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| Keywords: | chloroplast DNA broad-leaved tree species RFLP |
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