大鼠第三脑室室周区一氧化氮合酶阳性触液神经元的分布

目的：观察大鼠第三脑室室周区（3V-Pe)内一氧化氮合酶（NOS）阳性触液神经元（CSFCN）的形态及分布特征，为了解一氧化氮（NO）在脑-脑脊液神经体液回路中的调节作用提供形态学资料。方法：用黄递酶组织化学染色方法研究NOS神经元在大鼠3V-Pe的分布及其形态特点。结果：3V-Pe均有NOS-CSFCN分布，由前腹侧渐向后背侧过渡，细胞可分4种类型，位于室管膜内或其下层，或距之有一定的距离，但有突起伸向第三脑室，室旁核内有众多NOS阳性纤维向第三脑室伸展，与3V-PeNOS-CSFCN相互交错，邻接。结论：3V-PeNOS-CSFCN与脑脊液非常接近，提示它们自脑脊液获得信息，并能向脑脊液分泌NO，很可能具有神经-体液双方面的信息整合作用

The Distribution of Nitric Oxide Synthase Positive Cerebrospinal Fluid-contacting Neurons in the Surrounding Region of the Third Ventricle in the Rat

Objective:To observe the morphology and dis tribution o f nitric oxide synthase(NOS) positive cerebrospinal fluid(CSF) contacting neuro ns in the surrounding region of the third ventricle in the rat. Methods: NADPH diaphorase histochemistry method was used to investi ga te the distribution of NOS positive CSF contacting neurons in the surrounding r egion of the third ventricle in the rat. Results:NOS positive CSF contacting neurons were scattered at the s ur rounding region of the third ventricle, from anterior ventral area to posterior d orsal area. These neurons could be classified in four types. They were located i n the ependymal layer, subependymal layer. Some of them were located where there was some distance to the ependyma, but they had some processes protruded into t he third ventricle. In the anterior parts of posterior magnocellar paraventricular nuclei and medial magnocellar paraventricular nuclei, there were a large number of NOS positive fibers extending to the third ventricle, mixing with or contacti ng processes of NOS positive CSF contacting neurons. Conclusion: NO S positive CSF contacting neurons in the surrounding re gion of the third ventricle were very close to the CSF. So, we suggested that th ese neurons may acquire information from the CSF and release NO into the CSF, an d play an important role in neurohumoral regulation.