高浓度臭氧对蒙古栎叶片酚类物质含量和总抗氧化能力的影响

采用开顶箱(OTC)法，研究了高浓度臭氧(80±8 nmol·mol^-1)熏蒸处理对蒙古栎叶片 中总酚、类黄酮、单宁及丙二醛(MDA)含量的影响，使用1,1-二苯基苦基苯肼(DPPH) 法测定了蒙古栎叶片的总抗氧化能力.结果表明:蒙古栎叶片中总酚、类黄酮、缩合单宁和MDA的含量都有所增加，其中总酚和缩合单宁含量分别增加了48.17%和26.77%，差异显著（P<0.05）;类黄酮和MDA含量分别增加了24.66%和5.26%，差异不显著(P＞0.05);蒙古栎叶片总抗氧化能力显著增强(P<0.05)，且与叶片中总酚和缩合单宁含量呈显著的正相关关系.

Effects of elevated ozone on phenolic substances content and total antioxidative capacity of Quercus mongolica leaves.

An open-top chamber experiment was conducted to examine the effects o f elevated atmospheric O3 (80±8 nmol·mol-1) on the total phenols, flav onoid, condensed tannin, and MDA contents, and the total antioxidative capacity of Quercus mongolica leaves. Elevated O3 increased the contents of tota l phenols and condensed tannin by 4817% and 2677% (P<005), and the fla vonoid and MDA contents by 2466% and 526% (P>005), respectively. The t otal antioxidant capacity of Q. mogonlica leaves determined by DPPH method w as enhanced significantly (P<005), and had an obvious linear relationship with the contents of total phenols and condensed tannin.