基于磁性纳米颗粒微阵列技术的MT-2A基因启动子单核苷酸多态性分析

根据高通量检测手段对冶炼作业人群中金属硫蛋白MT-2A基因启动子核心区域进行单核苷酸多态性(SNP)分析.通过磁性纳米颗粒微阵列技术对冶炼作业工人(288例)的MT-2A基因启动子位点进行高通量SNP检测,并进行测序验证.结果表明:该冶炼作业人群中MT-2A基因启动子核心区域的3种基因型(AA,AG和GG)频率分别为87.4％,11.8％和0.8％,等位基因(A和G)频率分别为93.2％和6.8％.该人群MT-2A基因型与等位基因频率均符合Hardy-Weinberg遗传平衡检测(p＞0.05).最后,利用直接测序法对PCR产物进行测序,所得结果与SNP分析结果一致.

Analysis of Single Nucleotide Polymorphism in the Promoter of MT-2A Gene by Magnetic Nanoparticle-Based Microarray Technology

By high throughput detection means, the single nucleotide polymorphisms (SNPS) analysis is conducted on metallothionein MT-2A gene promoter core region of metallurgy operation population. The MT-2A gene promoter sites of metallurgy workers (288 cases) are carried on high throughput SNP detection by magnetic nanoparticles microarray technology, and the sequencing test is also made. The results show that three genotypes (AA, AG and GG) frequency of the MT-2A gene promoter core region in the workers are 87.4%, 11.8% and 0.8%, and alleles (A and G) frequency are 93.2% and 6.8%, respectively. The MT-2A genotype and allele frequencies are consistent with Hardy-Weinberg equilibrium (p>0.05). Finally applies the direct sequencing method for PCR product sequencing test, and the obtained results are consistent with the result of SNP analysis.