电磁辐射对大鼠心肌细胞Bcl-2和Bax表达的影响

目的 对比性研究3种波段电磁辐射对大鼠心肌细胞Bcl-2和Bax的表达变化,探讨其损伤心肌细胞凋亡的可能机制。方法 180只Wistar大鼠电脑随机分为对照组(C)、S波段微波辐照组(S)、X波段微波辐照组(X)、电磁脉冲辐照组(E),分别于辐照后6h、1d、3d、7d、14 d、28 d活杀,切取大鼠心脏标本行组织学、免疫组织化学检测Bcl-2和Bax的表达,应用图像分析技术,定量研究3种波段辐照后大鼠心脏组织Bcl-2和Bax的表达变化规律。结果 3种波段辐照后6h~7d大鼠心脏组织损伤呈加重趋势,心肌纤维排列紊乱,糖原颗粒减少,核染色质浓缩,蒲肯野纤维溶解,间质水肿,心肌间有浆液渗出;与对照组相比,心肌细胞凋亡增加,Bax和Bcl-2表达均增加(P<0.05),但Bax促凋亡表达强于Bcl-2的表达强度。14~28d后心脏呈修复恢复期,14~28d后各组Bcl-2和Bax蛋白表达比6h~7d有所减弱,Bcl-2表达强于Bax表达强度,但仍较对照组表达强。6~12个月心脏组织与对照组基本相似。结论 电磁辐射对心脏损伤过程中,心肌细胞凋亡起着重要的作用,并与凋亡蛋白Bax和Bcl-2表达改变有关,且高功率微波作用较电磁脉冲明显,而X的作用又较S明显。

A Study of the Expression of Bcl-2 and Bax in Rat Hearts Induced by Electromagnetic Radiations

Objective To study contrastly the variation of the expression of Bcl-2 and Bax protein in rat hearts induced by three kinds of Band Electromagnetic Radiations[X-band、S-band and Electromagnetic pluse（EMP） ]and investigate the possible mechanism of apoptosis.Methods 180 healthy Wistar rats were randomly divided into four groups：a control group（n=36） and three experimental groups（n=48） respectively treated with X-band、S-band high power microwave and EMP.At different time points（6 hours,1、3、7、14 、28 days,6 months,12 months）,the rats were killed after irradiated.We observed the pathological changes,examined the expression of Bcl-2 and Bax protein by using immunohistochemical technique and study the rule of change quantitatively by using imagine analysis technique.Results The injury of rat hearts was more and more serious at 6 hours-7 days：cardiomyofilaments were deranged;Glycogen were dicreased;Purkinje cells lysis;martix swelling and exudative serous.The expression of Bax and Bcl-2 protein was both stronger than the control group＇s（P0.05）.It means apotosis increase.The injured hearts showed resumptive at 14-28 days,and returned to normal progressively at 6-12 months.Conclusion Apoptosis might play an important role in the process of hearts injury induced by electromagnetic radiations and was relative to the expression of Bcl-2 and Bax protein.The injury grades are XSEMP.