HPLC法测定喘复康软胶囊中2组分含量

目的测定喘复康软胶囊中盐酸麻黄碱和盐酸异丙嗪的含量。方法HPLC法 ,采用shim packVp ODS色谱柱 ,流动相分别为甲醇 0 0 5moL·L-1磷酸二氢钾 三乙胺 (V∶V∶V =2 0∶80∶0 0 2和V∶V∶V =5 5∶4 5∶0 0 2 ,用磷酸调节 pH =3 4 ) ,检测波长分别为 2 0 6nm和 2 5 1nm。结果盐酸麻黄碱在 0 0 18～ 0 10 7mmol·L-1、盐酸异丙嗪在 0 0 14～ 0 0 37mmol·L-1内峰面积与浓度呈良好的线性关系 ,其回归方程分别为Y =3 2 13× 10 4X +2 83× 10 2 (r =0 9999)、Y =8 72 9× 10 4X +3 16 2× 10 3 (r =0 9999) ;回收率分别为 99 3% (RSD =0 6 % )和 99 4 % (RSD =0 6 % )。结论该方法可用于喘复康软胶囊中盐酸麻黄碱和盐酸异丙嗪 2种组分的含量测定

Determination of the contents of the two components in the Chuanfukang Soft Capsules by HPLC

Objective To determine the contents of the ephedrine hydrochloride and promethazine hydrochloride in the Chuanfukang Sofe Capsules with the C 18 column by HPLC.Methods The mobile phase was methanol- 0.05mol·L -1 KH 2PO 4-triethylamine (V∶V∶V=20∶80∶0.02)and (V∶V∶V=55∶45∶0.02)(pH=3.4). The peaks were detected at 206 nm and 251 nm. Result The calibration curves were liner in the range of 0.018～ 0.107 mmol·L -1 (r= 0.999 9) for ephedrine hydrochloride and 0.014～ 0.037 mmol·L -1 (r= 0.999 9) for promethazine hydrochloride. The average recovery was 99.3% (RSD=0.6%) and 99.4(RSD=0.6%).Conclusions The method is simple, fast, suitable for determination of the ephedrine hydrochloride and promethazine hydrochloride in the Chuanfukang Soft Capsules.