Increased insect resistance in transgenic wheat stably expressing trypsin inhibitor CMe |
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Authors: | Altpeter Fredy Diaz Isabel McAuslane Heather Gaddour Kamel Carbonero Pilar Vasil Indra K |
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Affiliation: | (1) Laboratory of Plant Cell and Molecular Biology, University of Florida, 1143 Fifield Hall, Gainesville, FL 32611-0690, USA;(2) Laboratorio de Bioquímica y Biología Molecular, Dpto. de Biotecnología-UPM, ETSI Agrónomos, 28040 Madrid, Spain;(3) Department of Entomology and Nematology, P.O. Box 110620, Gainesville, FL 32611-0620, USA |
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Abstract: | Proteinase inhibitors have been proposed to function as plant defence agents against herbivorous pests. We have introduced the barley trypsin inhibitor CMe (BTI-CMe) into wheat (Triticum aestivum L.) by biolistic bombardment of cultured immature embryos. Of the 30 independent transgenic wheat lines selected, 16 expressed BTI-CMe. BTI-CMe was properly transcribed and translated as indicated by northern and western blot, with a level of expression in transgenic wheat seeds up to 1.1% of total extracted protein. No expression was detected in untransformed wheat seeds. Functional integrity of BTI-CMe was confirmed by trypsin inhibitor activity assay. The significant reduction of the survival rate of the Angoumois grain moth (Sitotroga cerealella, Lepidoptera: Gelechiidae), reared on transgenic wheat seeds expressing the trypsin inhibitor BTI-CMe, compared to the untransformed control confirmed the potential of BTI-CMe for the increase of insect resistance. However, only early-instar larvae were inhibited in transgenic seeds and expression of BTI-CMe protein in transgenic leaves did not have a significant protective effect against leaf-feeding insects. |
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Keywords: | transgenic plants trypsin inhibitor insect resistance Triticum aestivum L |
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