大孔吸附树脂精制中药栀子中栀子苷的研究

研究了大孔吸附树脂精制中药栀子中栀子苷的工艺条件,以栀子苷含量为考察指标,比较了不同吸附树脂、吸附流速、洗脱流速等因素对精制栀子苷的影响,表明HPD-100树脂精制栀子苷的最佳工艺条件:吸附流速为2.0 mL/min,洗脱流速为1.0 mL/min,梯度洗脱,收集30%乙醇水溶液洗下来的洗脱液,干燥后,得到纯度为90.75%的栀子苷产品。此工艺可较好地精制中药栀子中的重要有效成分栀子苷,为工业化生产提供了方法依据。

Study on Adsorption and Purification of Geniposide with Macroporous Resin

To screen the optimum macroporous resin and technical conditions for the adsorption and purification of geniposide from the Chinese native medicine gardenia,the static and dynamic adsorption/desorption methods were used,and the separation and purification process was evaluated by measuring the concentration of geniposide by UV spectrophoto-meter.The HPD-100 macroporous resin was the most effective compared with other macroporous resins.The optimum conditions were screened as follows,The sample containing geniposide was passed through a column packed witi HPD-100 resin at the flow rate of 2.0 mL/min.After adsorp-tion,the resin was gradient-eluted by using aqueous ethanol solution at the flow rate of 1.0 mL/min.The eluate with 30% ethanol was collected and vacuum-distilled to remove ethanol and water and then was vacuum-dried to obtain a solid product.Under these conditions,a product with 90.75% of geniposide was obtained.It can be feasible to be utilized in industrial production of geniposide.