膜分离与大孔树脂联用技术纯化茶皂素

采用膜分离与大孔树脂联用技术纯化茶皂素。粗茶皂素经陶瓷膜和360Da纳滤膜初步分离浓缩,得率为62.1%,纯度为79%;根据静态和动态吸附筛选试验,选择大孔树脂AmberliteXAD7HP对茶皂素进一步纯化,通过单因素试验,确定最佳工艺参数为:上样流速0.5 mL/min、上样液浓度30mg/mL;以10%,40%,70%的乙醇溶液进行梯度洗脱,洗脱剂流速1mL/min,洗脱液体积为3BV,该条件下纯化,茶皂素最终得率为55.3%,纯度可达95%。该试验表明膜分离与大孔树脂联用技术可得到高纯度的茶皂素,是一种可工业化推广的方法。

Separation and purification of tea saponin by membrane separation combined with macroporous resin

The combination technology of membrane separation technology and macroporous resin purification technology have been adopted to separate and purify tea saponin. Tea saponin extractive was preliminarily purified and concentrated by flowing through ceramic membrane and 360 Da nanofiltration membrane in sequence, the yield and purity of tea saponin is 62.1% and 79%, respectively. On the basis of static adsorption and desorption as well as dynamic adsorption and desorption experiments, the macroporous resin AmberliteXAD7HP was selected for further purification. the The optimum conditions for purification of tea saponin using macroporous resin were established by single factor tests as follows: the sample flow rate of 0.5 mL/min, the sample concentration of 30 mg/mL; gradient elution with ethanol of different concentration, 10%, 40% and 70% respectively, flow rate of 1 mL/min, elution volume was 3 BV. Under these optimum conditions, the final yield of tea saponin was 55.3% with the purity of 95%. High purity tea saponin has been got, and indicates that membrane separation and macroporous resin combination technology is a feasible method of industrialization.