| 高产L-精氨酸谷氨酸棒状杆菌的构建 |
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| 引用本文： | 王婷,蔡柠匀,张德志,赵桂红,徐庆阳,陈宁.高产L-精氨酸谷氨酸棒状杆菌的构建[J].中国酿造,2019(5):169-173. |
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| 作者姓名： | 王婷 蔡柠匀 张德志 赵桂红 徐庆阳 陈宁 |
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| 作者单位： | 天津科技大学生物工程学院;天津科技大学代谢控制发酵技术国家地方联合工程实验室;天津科技大学教育部工业发酵微生物重点实验室 |
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| 摘 要： | 以产L-精氨酸诱变菌株谷氨酸棒状杆菌(Corynebacterium glutamicum)AJC为出发菌株,采用基因组编辑技术对其进行改造。首先,敲除阻遏蛋白ArgR和FarR,解除反馈阻遏作用;然后,敲除乳酸脱氢酶编码基因ldh和整合鸟氨酸乙酰转移酶编码基因argJ,阻断乳酸合成途径和增加前体物;最后,敲除谷氨酸分泌蛋白编码基因NCgl1221和整合乙酰谷氨酸激酶基因argB,减弱L-谷氨酸的胞外分泌,筛选一株L-精氨酸高产菌株。结果表明,获得一株高产L-精氨酸菌株AJC-4(C. glutamicum AJCΔargRΔfarRΔldh::PtufargJ ΔNCgl1221::PsodargB),该菌株在5 L发酵罐中发酵64 h后,L-精氨酸产量和糖酸转化率分别为78.0 g/L和0.38 g/g,较出发菌株AJC分别提高21.9%、18.8%;副产物乳酸和L-谷氨酸积累量分别为0.11 g/L、0.16 g/L,较出发菌株AJC分别降低96.8%、96.1%。
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| 关 键 词： | L-精氨酸 谷氨酸棒状杆菌 代谢工程 发酵 |
Construction of high yield L-arginine Corynebacterium glutamicum |
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| WANG Ting,CAI Ningyun,ZHANG Dezhi,ZHAO Guihong,XU Qingyang,CHEN Ning.Construction of high yield L-arginine Corynebacterium glutamicum[J].China Brewing,2019(5):169-173. |
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| Authors: | WANG Ting CAI Ningyun ZHANG Dezhi ZHAO Guihong XU Qingyang CHEN Ning |
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| Affiliation: | (College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China;National and Local United Engineering Lab of Metabolic Control Fermentation Technology,Tianjin University of Science and Technology,Tianjin 300457,China;Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,Tianjin University of Science and Technology,Tianjin 300457,China) |
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| Abstract: | WANG Ting;CAI Ningyun;ZHANG Dezhi;ZHAO Guihong;XU Qingyang;CHEN Ning(College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China;National and Local United Engineering Lab of Metabolic Control Fermentation Technology,Tianjin University of Science and Technology,Tianjin 300457,China;Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,Tianjin University of Science and Technology,Tianjin 300457,China) |
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| Keywords: | L-arginine Corynebacterium glutamicum metabolic engineering fermentation |
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