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红提葡萄中酵母菌多样性的研究
引用本文:徐建坤,张旺,肖婧,程卫东,史学伟. 红提葡萄中酵母菌多样性的研究[J]. 中国酿造, 2019, 38(3): 40. DOI: 10.11882/j.issn.0254-5071.2019.03.009
作者姓名:徐建坤  张旺  肖婧  程卫东  史学伟
作者单位:1.石河子大学 食品学院,新疆 石河子 832000;2.石河子大学 信息科学与技术学院,新疆 石河子 832000
基金项目:兵团科技攻关计划(2016AB009);兵团科技攻关计划(2016AD024)
摘    要:为研究红提葡萄酵母菌多样性,以石河子地区的红提葡萄为原料,采用传统分离培养方法从中筛选酵母菌,并通过形态 观察、生理生化试验及分子生物学技术对其进行鉴定。 结果表明,共筛选鉴定出24株酵母菌。 其中毕赤酵母属(Pichia)16株、汉逊 酵母属(Hanseniaspora)4株、梅奇酵母属(Metschnikowia)4株。 库德毕赤酵母(Pichia kudriavzevii)为优势种,占分离菌株的50%。 该 研究可为红提葡萄酵母菌资源的开发与利用提供理论依据。

关 键 词:红提葡萄  酵母菌  多样性  分离  鉴定  

Diversity of yeast in red grape
XU Jiankun,ZHANG Wang,XIAO Jing,CHENG Weidong,SHI Xuewei. Diversity of yeast in red grape[J]. China Brewing, 2019, 38(3): 40. DOI: 10.11882/j.issn.0254-5071.2019.03.009
Authors:XU Jiankun  ZHANG Wang  XIAO Jing  CHENG Weidong  SHI Xuewei
Affiliation:1.Food College, Shihezi University, Shihezi 832000, China; 2.College of Information Science and Technology, Shihezi University, Shihezi 832000, China
Abstract:In order to study the diversity of yeast in red grape, using the red grape in Shihezi as material, the yeasts were screened by traditional isola- tion and culture method and identified by morphologic observation, physiological and biochemical test and molecular biological technique. The re- sults showed that the 24 yeasts were screened and identified, 16 strains were Pichia, 4 strains were Hanseniaspora, and 4 strains were Metschnikowia. Pichia kudriavzevii was the dominant species, which accounted for 50%. The study provided a theoretical basis for the development and utilization of yeas resources.
Keywords:red grape  yeast  diversity  isolation  identification  
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