Factors Affecting the Development of Somatic Cell Nuclear Transfer Embryos in Cattle |
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Authors: | Satoshi AKAGI Kazutsugu MATSUKAWA Seiya TAKAHASHI |
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Affiliation: | 1)Animal Breeding and Reproduction Research Division, NARO Institute of Livestock and Grassland Science, Ibaraki 305-0901, Japan;2)Multidisciplinary Science Cluster, Life and Environmental Medicine Science Unit, Kochi University, Kochi 783-8502, Japan;3)Headquarters, National Agriculture and Food Research Organization, Ibaraki 305-8517, Japan |
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Abstract: | Nuclear transfer is a complex multistep procedure that includes oocyte maturation, cell cycle synchronization of donor
cells, enucleation, cell fusion, oocyte activation and embryo culture. Therefore, many factors are believed to contribute to
the success of embryo development following nuclear transfer. Numerous attempts to improve cloning efficiency have been
conducted since the birth of the first sheep by somatic cell nuclear transfer. However, the efficiency of somatic cell
cloning has remained low, and applications have been limited. In this review, we discuss some of the factors that affect the
developmental ability of somatic cell nuclear transfer embryos in cattle. |
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Keywords: | Cattle Cloning Embryo Reprogramming Somatic cell nuclear transfer |
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