白细胞介素6、8及肿瘤坏死因子α与子宫内膜异位症相关性的研究

目的　探讨子宫内膜异位症 (内异症 )患者血清和腹腔液中白细胞介素 6、8(IL -6、IL -8)、肿瘤坏死因子α(TNF -α)的变化及其在内异症发病中的作用。方法　采用双抗体夹心酶联免疫吸附试验测定 5 8例内异症患者和15例非内异症患者 (对照组 )血清和腹腔液中的IL -6、IL -8、TNF -α的含量 ,并分别与R -AFS评分进行相关性分析。结果　内异症患者血清和腹腔液中IL -6(分别为 :3 2 .77± 15 .2 3 pg/ml;12 6.79± 184.5 5 pg/ml)、IL -8(分别为 :5 3 .3 9± 43 .45pg/ml;15 5 .66± 190 .99pg/ml)、TNF -α(分别为 :3 1.0 9± 19.2 9pg/ml;3 5 .92± 2 2 .0 3pg/ml)明显高于对照组(血清 :17.5 9± 4.97pg/ml、18.15± 6.3 8pg/ml、11.15± 3 .71pg/ml;腹腔液 :2 1.5 1± 8.44 pg/ml、2 0 .15± 6.3 8pg/ml、12 .5 3± 7.15 pg/ml) (P <0 .0 1) ,且Ⅲ～Ⅳ期浓度升高。除腹腔液中IL -8外 (P >0 .0 5 ) ,内异症患者血清和腹腔液中IL -6、IL -8、TNF -α的浓度与R -AFS评分均呈正相关。内异症患者腹腔液中IL -6、IL -8的水平明显高于血清 (P<0 .0 1～ 0 .0 5 ) ,而腹腔液中TNF -α的水平与血清差异无显著性 (P >0 .0 5 )。结论　内异症患者血清和腹腔液中异常水平的IL -6、IL -8、TNF -α是血清和腹腔免疫动态?

Study on the correlation between interleukin 6,8 and tumor necrosis factor-alpha levels with endometriosis

Objective To investigate the levels of interleukin 6, 8 (IL-6, IL-8) and tumor necrosis factor-alpha (TNF-α) in serum andperitoneal fluid (PF) of patients with endometriosis (EM) and their role on pathogenesis of EM. Methods IL-6, IL-8 and TNF-α contents in serum and PF of 58 cases with EM were detected by enzyme linked immunoabsorbent assay and compared with the counterparts of 15 cases without EM(Controls).The correlation analyses between IL-6, IL-8, TNF-α concentrations in serum and PF ofEM patients and severity of EM were performed. Results The serum and PF from patients with EM contained significantly greater amounts ofIL-6(serum:32.77±15.23 pg/ml; PF:126.79±184.55 pg/ml)?IL-8(serun:53.39±43.45pg/ml, PF:155.66±190.99 pg/ml)?TNF-α(Serum:31.09±19.29 pg/ml, PF:35.92±22.03 pg/ml) than those in controls (Serum:17.59±4.97 pg/ml?18.15±6.38 pg/ml?11.15±3.71 pg/ml; PF:21.51±8.44 pg/ml, 20.15±6.38 pg/ml, 12.53±7.15 pg/ml, respectively) ( P <0.01). The highest serum and PF IL-6, IL-8, TNF-α concentration were found in stage Ⅲ～Ⅳ. Except IL-8 in PF ( r =0.249, P >0.05), significant relationship existed between the stages of EM and the IL-6, IL-8and TNF-α levels in serum and PF of EM patients (r values: serum:0.543,0.427,0.793; PF 0.695, 0.838, P <0.05～0.001). There were significantly higher levels of IL-6?IL-8 in PF than in serum of patients with EM ( P <0.01～0.05),but no difference was found in TNF-α between serum and PF ( P >0.05). Conclusion Increased levels of IL-6, IL-8 and TNF-α Levels inserum and PF account for imbalance of the immunologically dynamic environment inserum and peritoneal fluid of EM patients, which may be the cause of pathogenesis of EM.