蜘蛛香总黄酮大孔树脂纯化工艺

目的:研究蜘蛛香总黄酮的大孔树脂纯化工艺条件。方法:采用静态吸附-解吸方法,以吸附量和解吸率为指标,筛选最佳树脂;以总黄酮收率为指标,考察最佳树脂纯化蜘蛛香总黄酮的工艺参数。结果:5种树脂中,HPD600大孔树脂对蜘蛛香总黄酮纯化效果较好,其总黄酮的静态饱和吸附容量为131.11 mg.g-1干树脂,解吸率为91.69%;最佳动态吸附、洗脱条件为蜘蛛香总黄酮提取液质量浓度3.42 g.L-1,吸附流速2.4 BV.h-1,上样量25.64 mg.g-1干树脂;吸附后的树脂柱先以3BV水洗脱,再用4 BV 80%乙醇以2.4 BV.h-1流速洗脱,总黄酮质量分数为41.20%,收率为80.36%。结论:HPD600大孔树脂在所确定的工艺条件下,可较好的分离纯化蜘蛛香总黄酮。

Purification of Total Flavones from Valeriana Jatamansi by Macroporous Resins

ObJective: To study the optimal technologies for the purification of total flavones from Valeriana Jatamansi with macroporous resin. Method: Taking the static elution ratio and absorption ratio as indexes, the static absorption-desorption was used and compared in order to find the optimum macroporous resin.The technical process for purification of total flavones with the optimum macroporous resin was screened by yield of total flavones as index. Result: The HPD600 macroporous resin among five types of resins was found with the best separating efficiency, by which static absorption capacity and desorption ratio could reach 131.11 mg ·g^-1 and 91.69%, respectively. The best result of dynamic absorption was based on the followings: sample concentration 3.42 g ·L^-1,feed rate 2.4 BV ·h^-1, sample capacity 25.64 mg ·g^-1, elution flow rate 2.4 BV ·h^-1.After eluted with 3BV of distilled water and 4 BV of 80% ethanol, the yield of total flavones was 80.36% and the purity was 41.20%. Conclusion: With definite technical process, the HPD600 macroporous resin could be used to purify the total flavonoids of V. Jatamansi.