| 杜仲叶抗α-葡萄糖苷酶成分的研究 |
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| 引用本文： | 沈伊濛,王丽卫,文羽思,王仁涛,范俊峰.杜仲叶抗α-葡萄糖苷酶成分的研究[J].中国食物与营养,2014(1):58-61. |
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| 作者姓名： | 沈伊濛 王丽卫 文羽思 王仁涛 范俊峰 |
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| 作者单位： | [1]北京林业大学生物科学与技术学院／北京林业大学林业食品加工与安全北京市重点实验室,北京100083 [2]中国科学院过程工程研究所生化工程国家重点实验室,北京100190 |
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| 基金项目： | 2011年北京市大学牛科学研究与创业行动计划项目. |
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| 摘 要： | 通过正交试验优化了杜仲叶中抗α-葡萄糖苷酶成分的提取条件,并利用葡聚糖凝胶对杜仲叶水提液进行纯化分离,分析了杜仲叶水提物纯化液的黄酮含量。研究结果表明,杜仲叶的提取条件为提取料液比为1∶10,提取温度为90℃,提取时间为40min。杜仲叶水提物纯化液具有明显的抗α-葡萄糖苷酶效果,且杜仲叶中总黄酮含量为3.31%,纯化液中黄酮的含量为18.12%,杜仲总黄酮粗品得率为7.32%。
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| 关 键 词： | 杜仲叶 黄酮 α-葡萄糖苷酶 降血糖 |
Study on Anti-Alpha Glucosidase Components from Eucommia ulmoides Leaves |
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| SHEN Yi-meng,WANG Li-wei,WEN YU-xi,WANG Ren-tao,FAN Jun-feng.Study on Anti-Alpha Glucosidase Components from Eucommia ulmoides Leaves[J].Food and Nutrition in China,2014(1):58-61. |
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| Authors: | SHEN Yi-meng WANG Li-wei WEN YU-xi WANG Ren-tao FAN Jun-feng |
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| Affiliation: | 1 Beijing Key Laboratory of Forest Food Processing and Safety/College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China; 2 National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China) |
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| Abstract: | This paper studied the optimum extraction conditions of anti-alpha glucosidase components from Eucommia ulmoides leaves through the orthogonal experiment. Water extraction of Eucommia ulmoides leaves was separated and purified by Sephadex G-75, and the fla- vonoid content was analyzed. The optimal extraction conditions ot＇ Eucommia ulmoides leaves were ratio of solid to liquid 1：10 （w/v）, ex- traction temperature 90~C, and extracting time 40 min. The purified water extract of Eucommia ulmoides leaves had obvious anti-alpha gluco- sidas effect. The flavonoid content of Eucommia ulmoides leaves and the purified extract were 3.31% and 18.12% , respectively. The yield of crude flavonoids extract was 7.32% . |
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| Keywords: | Eucommia ulmoides leaves flavones α-glucosidase hypoglycemic |
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