细胞外基质在肝细胞性肝癌发生发展过程中的作用

肝细胞性肝癌（HCC）是我国常见的恶性肿瘤性疾病。近年来微环境对癌组织的影响受到重视，其中细胞外基质（ECM）作为HCC组织的重要微环境成分，在肿瘤发生发展过程中扮演着重要的角色，涉及肿瘤的生长、凋亡、耐药、侵袭、转移等。纤连蛋白（FN）、层粘连蛋白（LN）、透明质酸（HA）、胶原蛋白（COL）以及细胞外基质的硬度在HCC的发生发展过程中所起到的特殊作用。正是由于ECM各蛋白成分在HCC中的特殊作用，若盲目降解或敲除ECM中任意蛋白成分，可能无法达到预期的治疗效果，反而加速HCC的进展。所以需要探索某种特定的方法用于靶向调节ECM中成分，精准控制ECM重塑，从而调控HCC的发生发展。HCC微环境是一个持续变化的动态环境，为了尽可能模拟HCC细胞所处微环境，运用多种ECM蛋白成分而非单一成分联合培养HCC细胞以探究ECM多成分对细胞的共同作用是可行的研究方向。

Role of extracellular matrix in the occurrence and development of hepatocellular carcinoma

Hepatocellular carcinoma (HCC) is a common malignant tumor disease in China. In recent years, more and more attention has been paid to the influence of microenvironment on cancer tissue. Extracellular matrix (ECM), as an important microenvironment component of HCC tissue, plays an important role in tumor occurrence and development, involving tumor growth, apoptosis, drug resistance, invasion, metastasis and so on. The fibronectin (FN), laminin (LN), hyaluronic acid (HA), collagen (COL) and matrix stiffness play special roles in the tumorigenesis and progression of HCC. It is precisely because of the special role of each protein component of ECM in HCC, blindly degrading or knocking out any protein component in ECM may probably not be able to achieve the desired therapeutic effect, but accelerate the progress of HCC. Therefore, it is necessary to explore some specific drugs or methods for targeted regulation of ECM components, and purposeful control of ECM remodeling, thus affecting the occurrence and development of HCC. Considering that the microenvironment of HCC is a dynamically changing environment, it is a feasible research direction for exploring the co-action of ECM multi-components on HCC cells by co-culture of HCC cells with multiple ECM protein components instead of a single component to simulate the microenvironment of HCC cells as much as possible.