初诊脑瘫患儿外周血淋巴细胞亚群及血清免疫抗体、补体水平的变化

目的 分析初诊脑瘫患儿外周血淋巴细胞亚群及血清免疫抗体、补体水平,了解脑瘫患儿细胞及体液免疫状态.方法 选取2009年8月至2011年2月于本院小儿科首诊的脑瘫患儿69例,抽静脉血即时检测.应用四色流式细胞术结合CD45+设门分析外周血淋巴细胞亚群;采用速率散射比浊法方法检测血清IgA、IgG、IgM、总IgE及补体C3、C4.结果 与对照组相比,脑瘫患儿组外周血T淋巴细胞、NK细胞比例显著减少,B淋巴细胞比例显著增高(P<0.05);IgM、IgG水平显著增高,总IgE水平显著降低(P<0.05);其他差异均无统计学意义(P>0.05).结论 初诊脑瘫患儿存在细胞、体液免疫调节异常,可能与疾病早期免疫系统对受损中枢神经系统的保护作用有关.

Changes of blood lymphocyte subsets and serum antibody and complement in children with preliminary diagnosis of cerebral palsy

Objective To analyze the lymphocyte subsets and serum antibody and complement levels of children with preliminary diagnosis of cerebral palsy to understand the cellular and humoral immunity condition in them.Methods Blood samples of 69 children with preliminary diagnosis of cerebral palsy in our hospital from Aug 2009 to Feb 2010 were collected. Four-color flow cytometry were used for detecting the lymphocyte subsets by gating of CD45+.Rate nephelometry were used to measure serum IgA, IgG, IgM, total IgE and complement C3 and C4 level.Results Compared with control group, the percentage of T lymphocytes and NK cells in children with cerebral palsy reduced significantly, while that of B lymphocytes increased (P<0.05), serum IgM and IgG levels reduced significantly, while total IgE level increased (P<0.05), and other differences were not statistically significant(P>0.05)Conclusion There were abnormalities of cellular and humoral immune in children with preliminary diagnosis of cerebral palsy, which might be related with the protective effects of immune system to the damaged central nervous system at early stage of disease.