UPLC-MS/MS法测定氘代氯化琥珀胆碱在大鼠体内分布

目的:建立超高效液相色谱串联质谱(UPLC-MS/MS)法测定氘代氯化琥珀胆碱(2H-Suc)在大鼠主要脏器分布。方法:大鼠皮下注射2H-Suc 4 h后,用UPLC-MS/MS法测定血清和相关脏器中2H-Suc含量;色谱柱:Luna NH2色谱柱(2 mm×100 mm,3μm),流动相为0.1%甲酸水溶液-乙腈等度洗脱,流速为0.2 mL/min;正离子扫描模式,多反应监测模式下,电喷雾离子化源进行检测;考察该方法的线性关系与检测限、准确度和精密度、回收率、基质效应和稳定性。结果:血清和肾组织中2H-Suc的检测限分别是0.04 ng/mL和0.05 ng/mL,线性范围分别是0.3~100 ng/mL和0.5~100 ng/mL,日内、日间精密度和准确度均小于15%,回收率在85.30%~96.76%之间,经过处理后的样品不存在明显的基质效应。大鼠皮下注射2H-Suc,在血清及心、肝、肾等主要脏器中均检测到2H-Suc,其中肾脏中含量最高。结论:UPLC-MS/MS法是测定大鼠体内2H-Suc含量的一个有效方法,2H-Suc在大鼠血清及心、肝、肾等中均有分布,肾脏中含量最高。

Distribution of deuterosuccinylcholine chloride in rats determined by UPLC-MS/MS method

AIM:To establish an ultra-performance liquid chromatography-mass spectrometry(UPLC-MS/MS)method to determine the distribution of deuterosuccinylcholine chloride(2H-Suc)in rats.METHODS:After subcutaneous injection of 2H-Suc for 4 hours,contents of 2H-Suc in serum and related organs were determined by UPLC-MS/MS.A Luna NH2 column(2 mm×100 mm,3μm)was used for chromatography assay,the mobile phase was 0.1%formic acid aqueous solution-acetonitrile isolution,the flow rate was 0.2 mL/min;an electrospray ionization source was used to measure 2H-Suc in multi-reaction monitoring mode via positive ionization;the linear relationship,detection limit,accuracy and precision,recovery rate,matrix effect and stability of this method were investigated.RESULTS:The detection limit of Suc was 0.04 ng/mL in serum and 0.05 ng/mL in renal tissue,respectively;range of linear relationship was 0.3-100 ng/mL in serum and 0.5-100 ng/mL in renal tissue;intraday and interday precision and accuracy were less than 15%,the recovery was between 85.30%-96.76%,and there was no obvious matrix effect in the treated samples;2H-Suc was detected in serum,heart,liver,kidney and other organs of rats subjected to subcutaneous injection of 2H-suc for 4 h,and the highest content was found in kidney.CONCLUSION:UPLC-MS/MS analysis is an effective and reliable method for detecting 2H-Suc in rats.Heart,liver,kidney and other tissues are main organs distributed with 2H-Suc and its content in the kidney is the highest in rat.