调节剂对低温胁迫后恢复过程中棉花幼苗生长与生理特性的影响

【目的】棉苗经低温胁迫后喷施调节剂,研究不同植物生长调节剂对棉花幼苗低温冷害的修复作用,为棉花幼苗抵御低温冷害提供技术参考。【方法】以棉花品种新陆早57号为材料,采用人工模拟低温方法,在子叶期4℃ 24 h处理后,叶面喷施清水为对照,分别喷施7种不同植物调节剂溶液后,置于25℃ 14 h/10 h(昼/夜)的人工气候箱内恢复,分析不同生长调节剂对棉花幼苗冷害的生长情况和生理生化特征的影响。【结果】与清水处理比较,喷施植物生长调节剂后棉花幼苗的株高增加了5.10%~9.87%,茎粗增加了5.28%~8.71%,主根长增加了5.24%~9.33%,鲜重增加了2.26%~5.98%,干重增加了2.38%~8.61%,冷害指数下降了1.79~5.29个百分点,脯氨酸增加了5.04%~5.58%,可溶性蛋白含量增加了5.07%~5.67%,可溶性糖含量增加了5.05%~5.87%;CAT酶活性增加了2.21%~7.95%。SOD酶活性增加了3.79%~5.56%,POD酶活性增加了2.18%~5.56%。【结论】在低温胁迫后,在常温恢复过程中叶面喷施生长调节剂可有效的提高棉花幼苗叶片的渗透调节能力和抗氧化能力,促进植株生长,修复低温胁迫对植株的伤害,以植物生长调节剂处理提高棉花幼苗抗寒性。其中,调节剂对低温胁迫后棉花幼苗恢复效果的综合作用排序为“阿泰灵”>α-萘乙酸钠>胺鲜脂>“碧护”>复硝酚钠>腐殖酸>黄腐酸钾Ⅱ型>对照。

Effects of Regulators on the Growth and Physiological Characteristics in Cotton Seedling during Restoration after Chilling Stress

【Objective】 After chilling stress, cotton seedlings were sprayed with plant growth regulators to explore the repair effects of different plant growth regulators on chilling stress of cotton seedlings, so as to provide technical reference for cotton seedlings to resist chilling. 【Methods】 The cotton variety 'Xinluzao 57' was treated at 4℃ for 24 hours at the cotyledon stage by artificial simulated chilling environment. The leaves were sprayed with clear water as the control, and 7 different plant regulator solutions were sprayed respectively. The effects of growth regulators on the growth and physiological and biochemical characteristics of cotton seedlings during restoration after chilling Stress were analyzed. 【Results】 The results showed that compared with the control, after spraying growth regulator, the plant height of cotton seedlings increased by 5.10% - 9.87%, stem diameter increased by 5.28% - 8.71%, root length increased by 5.24% - 9.33%, fresh weight increased by 2.26% - 5.98%, dry weight increased by 2.38% - 8.61%, chilling injury index decreased by 1.79% - 5.29%, the content of proline increased by 5.04% - 5.58%, the content of soluble protein increased by 5.07% - 5.67%, the content of soluble sugar increased by 5.05% - 5.87%, SOD activity increased by 3.79%-5.56%, POD enzyme activity increased by 2.18%-5.56%, CAT enzyme activity increased by 2.21% - 7.95%.【Conclusion】 After chilling stress, spraying growth regulators on the leaves during the recovery of normal temperature can effectively improve the osmotic adjustment ability and antioxidant ability of cotton seedling leaves, promote plant growth and repair the damage to the plant caused by chilling stress. This is a convenient and practical agronomic measure to improve the resistance of cotton to chilling. For the cotton area in Xinjiang where chilling damage occurs frequently in early spring, the technology of using plant growth regulator to improve the chilling resistance of cotton seedlings has a certain application prospect. Among them, the comprehensive effects of regulators on the restoration of cotton seedlings after chilling stress are as follows: "ATAILIN" > α- Sodium naphthylacetate >Diethyl aminoethyl hexanoate> "BIHU" > Compound Sodium Nitrophenolate > Humic acid >Potassium fulvic acid type II >Control.