附子中脂类生物碱的电喷雾三重四极杆质谱研究

附子是常用的中药药物，其中含有的脂类生物碱是附子中重要的活性成分。为了对附子中脂类生物碱的复杂体系进行快速分析，以附子中脂类生物碱为研究对象，建立一种稳定、高效、可靠的分析方法，本研究利用电喷雾三重四极杆质谱的多种扫描功能（全扫描、子离子扫描、母离子扫描、中性丢失扫描）分析附子中脂类生物碱。脂类生物碱间的差别分两种，其一是由于生物碱骨架不同导致的差别；其二是由于C8位所连接的脂肪酸的不同导致的差别。根据这两种差别，分析出附子中含量最多的4类生物碱骨架以及3类C8位取代脂肪酸，共12种脂类生物碱。三重四极杆质谱的母离子扫描及中性碎片丢失扫描功能用于分析附子中脂类生物碱，扫描速度快、效率高、一次扫描同时确定多种结构，所得结果稳定、可靠。

Analysis of the Lipo-Alkaloids from Aconite Root by Electrospray Triple Quadrupole Mass Spectrometry

Aconite tuber is one of the commonly used Chinese traditional medicine.Lipo-alkaloids is the most important active ingredients in Aconite tuber.A stable,efficient and reliable method was established to quickly analysed the lipo-alkaloids which are rather complex in aconite tuber.The lipo-alkaloids in aconite tuber is analysed by using multiple scan function of electrospray ionization coupled with triple quadrupole mass spectrometry including full scan,daughter ion scan,parent ion scan and neutral loss scan.There are two kinds of differences in the lipo-alkaloidal structures,one in which base on the different alkaloid skeleton,the other difference is due to the connected with C8 substituted long chain fatty acids.The lipo-alkaloids with the high content in aconite tuber are due to 4 kinds of alkaloids skeleton and 3 kinds of C8 substituted fatty acids,e.g.a total of 12 kinds of lipo-alkaloids which are analysed.People often only focus on the much reaction monitoring(MRM) function of the triple quardrupole mass spectrometry,ignore the other functions.The function of parent ion scan and neutral loss scan for analysis of the lipo-alkaloids from aconite root has scanning speed,high efficiency.The results are stable and reliable.