雌二醇对去势SD大鼠糖脂代谢影响的研究

目的探讨戊酸雌二醇灌胃给药对去势雌性SD大鼠血糖、血脂水平的影响。方法将90只4月龄雌性SD大鼠随机分为正常对照组、假手术组、去势对照组及药物组,药物组进行戊酸雌二醇灌胃给药;14周后腹主动脉采血,测定血清雌二醇(E2)及空腹血糖(FBG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、总胆固醇(TC)、三酰甘油(TG)水平。结果药物组血清E2水平明显高于去势对照组、假手术组及正常对照组(P<0.05),去势组血清E2水平明显低于正常对照组及假手术组(P<0.05);药物组HDL-C水平明显高于去势对照组(P<0.05),LDL-C水平明显低于去势对照组(P<0.05),但与正常对照组及假手术组差异均无统计学意义(P>0.05);去势对照组HDL-C和LDL-C水平与正常组及假手术组比较,差异均无统计学意义(P>0.05);各组间TG、TC及FBG水平差异无统计学意义(P>0.05)。结论口服戊酸雌二醇可以改善血脂,升高去势大鼠HDL-C及降低LDL-C水平。

Study on the Effect of Estrogen on the Glucolipide in Ovariectomized SD Rats

Objective To investigate the effects of estrogen on the glucolipide metabolism in ovariectomized SD rats.Methods Ninety SPF female SD rats,four-month-old,were randomly divided into four groups:normal control group,sham operation group,ovariectomized control group and treatment group.The treatment group were given intragastric administration of estradiol valerate,once per day,the other three groups were given the intragastric administration of physiological saline,once per day.Fourteen weeks after the treatment,animals′ blood samples were taken from aorta to assess the levels of estradiol(E2),fasting blood glucose(FBG),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),total cholesterol(TC) and triglyeride(TG).Results Fourteen weeks after intragastric administration,serum E2 level in the treatment group was significantly higher than that in the ovariectomized control group,normal control group and sham-operation group(P<0.05).Serum E2 level in the ovariectomized control group was significantly lower than that in normal control group and sham-operation group(P<0.05);HDL-C level in treatment group was significantly higher than that in ovariectomized control group(P<0.05),while LDL-C level was significantly lower than that in ovariectomized control group(P<0.05),there was no statistically significant differences in the above-mentioned indicators when compare the treatment group with normal control group and sham-operation group(P>0.05);in ovariectomized control group,HDL-C level was significantly lower than that in normal control group and sham-operation group without statistically significant differences(P>0.05),while LDL-C level was higher than that in normal control group and sham-operation group without statistically significant differences(P>0.05);there was no statistically significant differences between each group in TG,TC and FBG(P>0.05).Conclusion Estrogen valerate by intragastric administration might improve the blood lipids,elevate high density lipoprotein and reduce low density lipoprotein level.