火炭母提取物对金黄色葡萄球菌的抑菌活性及稳定性研究

目的：研究火炭母提取物对金黄色葡萄球菌的抑菌活性及稳定性。方法：用牛津杯法和微量肉汤稀释法来测定火炭母提取物的体外抑菌活性，并以金黄色葡萄球菌的OD_{600 nm}值为指标，进一步探讨温度、pH、紫外线及Na⁺、K⁺、Ca²⁺等金属离子对其抑菌稳定性的影响。结果：火炭母提取物对金黄色葡萄球菌具有一定抑菌活性，最小抑菌浓度为8 mg/mL；火炭母提取物在热处理、不同pH、紫外线照射等情况下抑菌活性较为稳定；当添加不同金属离子时其抑菌活性具有明显差异，0.1 mol/L Na⁺、K⁺处理时其抑菌活性有所下降，而在0.1 mol/L Ca²⁺处理时其抑菌活性丧失。结论：火炭母提取物对金黄色葡萄球菌具有稳定的体外抑菌活性。

Antibacterial activity and stability of Polygonum chinense Linn extracts on the Staphylococcus aureus

Objective: To study the antibacterial activity and stability of Polygonum chinense Linn extracts against standard strains of Staphylococcus aureus in vitro. Methods: The antibacterial activity of Polygonum chinense Linn extracts on the Staphylococcus aureus were determined by oxford cup test and microdilution broth method. The OD_{600 nm} value of S. aureus was used as an index, and the influence of pH, temperature, ultraviolet radiation and metal ions on its stability was explored. Results: The results showed that Polygonum chinense Linn extracts had certain inhibitory activity on Staphylococcus aureus, and the minimum inhibitory concentration was 8 mg/mL. In addition, Polygonum chinense Linn extracts had good antibacterial stability in the presence of heat treatment, acidic and basic condition, ultraviolet irradiation, but it was sensitive to metal ions. The antibacterial effect of metal ions on the Polygonum chinense Linn extracts was different,0.1 mol/L Na⁺ and K⁺ could reduce its antibacterial effect, but under the treatment of 0.1 mol/L Ca²⁺, the antibacterial activity was lost. Conclusion: Polygonum chinense Linn extracts had stable inhibitory activity against S. aureus in vitro.