| 直投式发酵剂制备中乳酸菌增殖条件的探讨 |
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| 引用本文： | 姜铁民,隋欣,王建,陈历俊,周伟明. 直投式发酵剂制备中乳酸菌增殖条件的探讨[J]. 食品与发酵工业, 2005, 31(8): 112-114 |
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| 作者姓名： | 姜铁民 隋欣 王建 陈历俊 周伟明 |
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| 作者单位： | 北京三元食品股份有限公司,北京,102206 |
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| 摘 要： | 为获得高浓度的乳酸菌细胞,对化学中和法培养乳酸菌的增殖条件进行了研究。主要采用菌落计数和显微镜检的方法,确定乳酸菌增殖的最佳温度、pH值及菌种中球菌与杆菌的比例。结果表明,在优化条件下,即32℃、pH5.8及球菌与杆菌比例为1∶2的培养条件下,乳酸菌活菌数达3.2×1010cfu/mL。
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| 关 键 词： | 直投式发酵剂 化学中和法 乳酸菌 增殖 |
| 收稿时间： | 2005-05-16 |
| 修稿时间： | 2005-06-23 |
Discuss on the Propagation of Lactic Acid Bacteria for Preparation of Direct Vat Inoculation |
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| Jiang Tiemin,Sui Xin,Wang Jian,Chen Lijun,Zhou Weiming. Discuss on the Propagation of Lactic Acid Bacteria for Preparation of Direct Vat Inoculation[J]. Food and Fermentation Industries, 2005, 31(8): 112-114 |
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| Authors: | Jiang Tiemin Sui Xin Wang Jian Chen Lijun Zhou Weiming |
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| Abstract: | In order to prepare concentrated bacterial cells, the effects of culture conditions on the propagation of lactic acid bacteria with chemical neutralization were studied. The optimum propagation conditions, including temperature, pH and the ratio of Lactococcus to Lactobacillus were obtained using colony enumeration method and microscope. The results showed that the number of viable cells was about 3.2×10 10 cfu/mL when the conditions were as follows:temperature 32℃, pH5.8, Lactococcus∶Lactobacillus=1∶2. The selected conditions were best for propagation of lactic acid bacteria. |
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| Keywords: | chemical neutralization lactic acid bacteria propagation |
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