具有益生特性植物乳杆菌的筛选及其发酵特性的研究

通过体外益生特性和安全性评价试验,从4株植物乳杆菌中筛选出益生特性较好的益生菌菌株B14,并对B14的发酵特性进行研究。结果表明,植物乳杆菌B14与其它菌株相比,其对二甲苯和十六烷的疏水率达到80%以上,自聚集能力超过85%,共聚集能力超过75%,表现出更好的益生特性,同时,B14对Caco-2细胞的黏附能力达到30.756%,且初步判定为安全菌株。将植物乳杆菌B14作为辅助发酵剂添加在发酵乳中,24 h内发酵乳的黏度、滴定酸均得到了一定的提高。表明植物乳杆菌B14具有作为发酵乳益生菌辅助发酵剂的潜在应用前景。

Screening of Lactobacillus plantarum with Probiotic Properties and Its Fermentation Characteristics

The strains with better prebiotic characteristics were screened out from four Lactobacillus plantarum strains by in vitro prebiotic characteristics as well as safety evaluation tests,and the fermentation characteristics of selected strains were studied. The results showed that compared with other strains,the hydrophobic rate of p-xylene and cetane of L. plantarum B14 was more than 80%,the auto-aggregation ability was more than 85%,and the co-aggregation ability was more than 75%. At the same time,the adhesion ability of B14 to Caco-2 cells reached 30.756%and B14 was preliminarily determined as a safe strain. When L. plantarum B14 was added to the fermented milk as an auxiliary starter,the viscosity and titrated acid of the fermented milk were improved in 24 hours. It indicated that L. plantarum B14 would have potential application prospects as an auxiliary starter for fermented milk.