响应面法优化超声辅助提取杜仲叶中的绿原酸

采用超声辅助法对杜仲叶中绿原酸的提取工艺进行研究。在单因素试验的基础上,采用Box-Benhnken中心组合进行三因素三水平的试验设计,通过回归分析各工艺参数与响应值之间的关系,并由此预测最佳的工艺条件。绿原酸含量通过高效液相色谱法(high performance liquid chromatography,HPLC)测定。结果表明,杜仲叶绿原酸最佳的提取工艺条件为:提取时间75 min,甲醇体积分数70%,超声功率270 W,提取温度45℃,绿原酸的提取得率预测值为2.532%,而在此试验条件下,绿原酸提取得率为2.529%。

Optimization of Ultrasound-assisted Extraction of Chlorogenic Acid from Eucommia ulmoides Leaves by Response Surface Methodology

Ultrasonic assisted method was used to investigate the exetraction of chlorogenic acid from Eucommia ulmoides leaves.On the basis of the single factor experiment,the box-benhnken center combination was used to carry out the three-factor and three-level experimental design,and the relationship between the process parameters and the response values was analyzed by regression,and the optimal process conditions were predicted.The chlorogenic acid content was determined by high performance liquid chromatography.The results showed that the optimal extraction conditions of chlorogenic acid from Eucommia ulmoides Oliver were as follows extraction time 75 min,methanol volume fraction 70%,ultrasonic power 270 W,extraction temperature45℃,and the predicted yield of chlorogenic acid was 2.532%.Under the experimental conditions,the extraction yield of chlorogenic acid was 2.529%.