绣球菌低分子量多糖的制备工艺优化及其分子量的测定

为研究绣球菌低分子量多糖的制备工艺,本文考察硫酸浓度、反应时间和料液比对多糖水解度的影响,以绣球菌多糖水解度为响应值,通过二次项数学模型拟合出最优酸解工艺参数,并采用多角度激光光散射仪测定酸解前后多糖的分子量。结果表明:在硫酸浓度0.46 mol/L、反应时间85 min、液料比6:1 mL/,绣球菌多糖水解度为67.06%±0.77%,与预测值接近,模型拟合效果良好。绣球菌多糖含有3个组分,重均分子量分别为3.32×107、1.79×106和3.18×106 Da,酸解后得到一种低分子量绣球菌多糖,含两个组分,重均分子量分别为3.25×105、5.61×105 Da,表明该法成功制备了一种绣球菌低分子量多糖。为后续的绣球菌低分子量多糖分离纯化和分子量-生物活性关系的研究提供了理论基础。

Optimization of Preparation Process and Molecular Weight Determination of Low Molecular Weight Polysaccharide from Sparassis crispa

In order to study the preparation process of Sparassis crispa low molecular weight polysaccharide,the effects of sulfuric acid concentration,acid hydrolysis time and sulfuric acid volume on the degree of hydrolysis of polysaccharide were investigated.The hydrolysis degree of Sparassis crispa polysaccharide was used as the response value and fitted by the quadratic mathematical model.The optimum acid hydrolysis process parameters were used,and the molecular weight of polysaccharides before and after acid hydrolysis were determined by multi-angle laser light scattering. The results showed that in the sulfuric acid concentration of 2.5%,the reaction time of 85 min,the liquid to material ratio of 6 ∶ 1 m L/g,the Sparassis crispa polysaccharide hydrolysis degree was 67.06% ± 0.77%,which was close to the predicted value,and the model fitting effect was good. Sparassis crispa polysaccharide contained three components,and the weight average molecular weight were 3.32 × 10^7,1.79 × 10^6 and 3.18 × 10^6 Da,respectively. After acid hydrolysis,Sparassis crispa low molecular weight polysaccharide got two groups.The weight average molecular were 3.25 × 10^5 and 5.61 × 10^5 Da,respectively,and the components were highly uniform before and after acid hydrolysis. This indicated that the preparation of low molecular weight polysaccharides was successful,which would provide a theoretical basis for subsequent separation and purification and biological activity research.